Molecular evolutionary analysis of the widespread piggyBac transposon family and related "domesticated" sequences
Molecular evolutionary analysis of the widespread piggyBac transposon family and related "domesticated" sequences
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DOI：
10.1007/s00438-003-0909-0
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发表时间：
2003-10-01
影响因子：
3.1
通讯作者：
Collins, FH
中科院分区：
文献类型：
--
作者：
Sarkar, A;Sim, C;Collins, FH
PiggyBac 是一种短的反向重复型 DNA 转座元件，最初是从蛾 Trichoplusia ni 的基因组中分离出来的。它是目前多种昆虫物种转化的首选基因载体。一些与piggyBac相似的序列先前已在人类（Looper）、河豚红鳍东方鲀（Pigibaku）、非洲爪蟾（Tx）、水蚤（Pokey）和东方果蝇橘小实蝇等生物体中鉴定出。我们现在已经从公开的基因组序列和表达序列标签 (EST) 中鉴定出 50 个 PiggyBac 样序列。这项调查首次对独特的piggyBac转座酶进行了比较检查，表明它可能包含一个高度分化的DDD结构域，与许多DNA转座酶和逆转录病毒整合酶中发现的广泛的DDE结构域相当，该结构域由两个绝对保守的天冬氨酸组成，相距约70个氨基酸，与一个高度保守的谷氨酸相距约35个氨基酸。在系统发育多样化的生物体的基因组中发现了许多piggyBac样序列，包括真菌、植物、昆虫、甲壳类动物、尾索动物、两栖动物、鱼类和哺乳动物。此外，还鉴定了宿主基因组对piggyBac转座酶序列“驯化”以实现细胞功能的几个实例。 PiggyBac家族的新成员可能在许多生物体的基因工程中有用。
piggyBac is a short inverted-repeat-type DNA transposable element originally isolated from the genome of the moth Trichoplusia ni. It is currently the gene vector of choice for the transformation of various insect species. A few sequences with similarity to piggyBac have previously been identified from organisms such as humans (Looper), the pufferfish Takifugu rubripes (Pigibaku), Xenopus (Tx), Daphnia (Pokey), and the Oriental fruit fly Bactrocera dorsalis. We have now identified 50 piggyBac-like sequences from publicly available genome sequences and expressed sequence tags (ESTs). This survey allows the first comparative examination of the distinctive piggyBac transposase, suggesting that it might contain a highly divergent DDD domain, comparable to the widespread DDE domain found in many DNA transposases and retroviral integrases which consists of two absolutely conserved aspartic acids separated by about 70 amino acids with a highly conserved glutamic acid about 35 amino acids further away. Many piggyBac-like sequences were found in the genomes of a phylogenetically diverse range of organisms including fungi, plants, insects, crustaceans, urochordates, amphibians, fishes and mammals. Also, several instances of 'domestication' of the piggyBac transposase sequence by the host genome for cellular functions were identified. Novel members of the piggyBac family may be useful in genetic engineering of many organisms.