Initial characterization of a bolA homologue from Pseudomonas fluorescens indicates different roles for BolA-like proteins in P-fluorescens and Escherichia coli
Initial characterization of a bolA homologue from Pseudomonas fluorescens indicates different roles for BolA-like proteins in P-fluorescens and Escherichia coli
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DOI：
10.1111/j.1574-6968.2006.00359.x
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发表时间：
2006-09-01
影响因子：
2.1
通讯作者：
Nybroe, Ole
中科院分区：
文献类型：
--
作者：
Koch, Birgit;Nybroe, Ole
大肠杆菌中RpoS调控的bolA基因对于稳定期或突然碳饥饿期间细胞大小的减小是重要的。一个荧光假单胞菌菌株突变的基因同源bolA减少其细胞大小后碳饥饿，和RpoS bolA表达的影响不大。在以L-丝氨酸为唯一氮源的基本培养基中，突变株的生长速度比野生型菌株慢，而在L-丝氨酸和L-半胱氨酸的混合物中生长速度相似。逆转录聚合酶链反应分析表明，bolA同源物是操纵子中的第二个基因，其中两个下一个ORF编码与硫转移酶和蛋白质二硫化物异构酶同源的推定蛋白质。突变体表型的互补仅通过编码BolA以及上述两种蛋白质的质粒获得。生长表型和基因同源性表明BolA样蛋白在大肠杆菌中具有不同的功能。大肠杆菌和假单胞菌属。
The RpoS-regulated bolA gene in Escherichia coli is important for the decrease in cell size during stationary phase or sudden carbon starvation. A Pseudomonas fluorescens strain mutated in a gene with homology to bolA reduced its cell size upon carbon starvation, and RpoS had little effect on bolA expression. The mutant grew slower than the wild-type strain in minimal medium with L-serine as the sole nitrogen source, while growth rates were similar on a mixture of L-serine and L-cysteine. Reverse transcriptase polymerase chain reaction analysis indicated that the bolA homologue is the second gene in an operon where the two next ORFs encode putative proteins with homology to sulphurtransferases and protein disulphide isomerases. Complementation of the mutant phenotypes was only obtained by plasmids encoding BolA as well as the above two proteins. Growth phenotypes and gene homologies suggest that BolA-like proteins have different functions in E. coli and Pseudomonas.