Volatilomes of Cyclocybe aegerita during different stages of monokaryotic and dikaryotic fruiting
Volatilomes of Cyclocybe aegerita during different stages of monokaryotic and dikaryotic fruiting
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DOI：
10.1515/hsz-2019-0392
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发表时间：
2020-07-01
影响因子：
3.7
通讯作者：
Ruehl, Martin
中科院分区：
文献类型：
--
作者：
Orban, Axel;Hennicke, Florian;Ruehl, Martin
挥发性有机化合物（VOC）是不同真菌物种的特征。然而，人们对VOC在发育过程中的变化及其生物学作用知之甚少。因此，我们建立了一个实验室培养系统，在改进的结晶皿中分析挥发性有机化合物的子实体发育过程中的双孢菌株Cyclocybe aegerita AAE-3以及四个单核后代兄弟姐妹表现出不同的出菇表型。从这些中，挥发性有机化合物直接从顶空（HS）中提取，并通过气相色谱-质谱（GC-MS）进行分析。在所有供试菌株的早期发育阶段，醇和酮，包括辛-1-烯-3-醇，2-甲基丁-1-醇和环戊酮，是HS的优势物质。在双核体中，VOC的组成随着子实体的发育而改变，甚至在孢子形成期间更剧烈。在生长后期，倍半萜类化合物，尤其是δ（6）-原伊鲁烯、α-立方烯和δ-杜松烯是主要成分。孢子形成后，倍半萜类化合物的量减少，而额外的挥发性有机化合物，主要是辛烷-3-酮，出现。在单核体的HS中，存在较少的VOC，其全部在双核体C的HS中可检测到。AAE-3.研究结果表明，香樟挥发性物质组的组成比香樟挥发性物质组的组成要高。Aegerita根据子实体的发育阶段而变化很大。
Volatile organic compounds (VOC) are characteristic for different fungal species. However, little is known about VOC changes during development and their biological role. Therefore, we established a laboratory cultivation system in modified crystallizing dishes for analyzing VOC during fruiting body development of the dikaryotic strain Cyclocybe aegerita AAE-3 as well as four monokaryotic offspring siblings exhibiting different fruiting phenotypes. From these, VOC were extracted directly from the headspace (HS) and analyzed by means of gas chromatography-mass spectrometry (GC-MS). For all tested strains, alcohols and ketones, including oct-1-en-3-ol, 2-methylbutan-1-ol and cyclopentanone, were the dominant substances in the HS of early developmental stages. In the dikaryon, the composition of the VOC altered with ongoing fruiting body development and, even more drastically, during sporulation. At the latter stage, sesquiterpenes, especially Delta(6)-protoilludene, alpha-cubebene and delta-cadinene, were the dominant substances. After sporulation, the amount of sesquiterpenes decreased, while additional VOC, mainly octan-3-one, appeared. In the HS of the monokaryons, less VOC were present of which all were detectable in the HS of the dikaryon C. aegerita AAE-3. The results of the present study show that the volatilome of C. aegerita changes considerably depending on the developmental stage of the fruiting body.