Application of preparative high-speed counter-current chromatography/electrospray ionization mass spectrometry for a fast screening and fractionation of polyphenols
Application of preparative high-speed counter-current chromatography/electrospray ionization mass spectrometry for a fast screening and fractionation of polyphenols
复制标题
DOI：
10.1016/j.chroma.2007.09.066
复制
发表时间：
2007-11-16
影响因子：
4.1
通讯作者：
Jerz, Gerold
中科院分区：
文献类型：
--
作者：
Gutzeit, Derek;Winterhalter, Peter;Jerz, Gerold
本研究提出了一种新的制备高速逆流色谱-电喷雾质谱（HSCCC/ESI-MS-MS）联用技术，用于沙棘浓缩汁中多酚类化合物的连续质谱数据采集和目标导向分离。SSP.鼠李属，胡颓子科）。结果表明，HSCCC和ESI-MS-MS的组合是一种通用的工具，用于快速筛选和灵敏检测粗提物中的生物活性天然产物。通过了解完整HSCCC分离的连续MS数据，将简化样品分馏。在分离过程中，已经提供了分离化合物的直接结构信息。HSCCC的峰纯度可通过完整的离子迹线进行评估，并且馏分可直接蒸发用于进一步的一维/二维NMR分析。(c)2007 Elsevier B. V.保留所有权利。
This study presents the novel direct coupling of preparative high-speed counter-current chromatography with electrospray ionization mass spectrometry (HSCCC/ESI-MS-MS) for continuous MS data acquisition, and 'target'-guided fractionation of polyphenolic compounds from sea buckthorn juice concentrate (Hippophae rhamnoides L. ssp. rhamnoides, Elaeagnaceae). It was demonstrated that a combination of HSCCC and ESI-MS-MS is a versatile tool for fast screening and sensitive detection of bioactive natural products in crude extracts. Sample fractionation will be simplified by knowing the continuous MS data of the complete HSCCC separation. Already during the separation, immediate structural information of the separated compounds is provided. Peak purity of HSCCC is assessable by complete ion traces and fractions can directly be evaporated for further one/two-dimensional NMR analysis. (c) 2007 Elsevier B.V. All rights reserved.