Brn-3a deficiency increases tyrosine hydroxylase-immunoreactive neurons in the dorsal root ganglion.
Brn-3a deficiency increases tyrosine hydroxylase-immunoreactive neurons in the dorsal root ganglion.
复制标题
Brn-3a 缺乏会增加背根神经节中酪氨酸羟化酶免疫反应性神经元。
DOI：
10.1016/j.brainres.2004.10.028
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Sugimoto,Tomosada
中科院分区：
文献类型：
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作者：
Ichikawa,Hiroyuki;Mo,Zeqian;Xiang,Mengqing;Sugimoto,Tomosada
酪氨酸羟化酶（TH）的免疫组织化学进行野生型，杂合子和Brn-3a基因敲除小鼠在胚胎第18.5天的背根神经节（DRG）。TH-免疫反应（-IR）神经元中检测到的野生型和杂合子小鼠的背根节，但其比例大大增加的Brn-3a功能的损失（野生型和杂合子，8.4%;敲除，20.9%）。野生型IR神经元的大小不同（平均值± SD = 118.1 ± 55.4 μm2，范围= 26.6-306.3 μm2）和杂合子小鼠。然而，在基因敲除小鼠中，TH-1 R神经元大多较小（平均值± S.D. = 68.2 ± 34.3 μm2，范围= 11.8-166.8 μm2）。目前的研究表明，Brn-3a可能在正常情况下抑制许多小DRG神经元中的TH表达，但激活大DRG神经元中的TH表达。
Immunohistochemistry for tyrosine hydroxylase (TH) was performed on the dorsal root ganglia (DRG) in wild-type, heterozygous and Brn-3a knockout mice at embryonic day 18.5. TH-immunoreactive (-IR) neurons were detected in the DRG of wild-type and heterozygous mice, but their proportion was greatly increased by the loss of Brn-3a function (wild-type and heterozygot, 8.4%; knockout, 20.9%). IR neurons were of various sizes in wild-type (mean ± S.D. = 118.1 ± 55.4 μm2, range = 26.6–306.3 μm2) and heterozygous mice. In the knockout mice, however, TH-IR neurons were mostly small (mean ± S.D. = 68.2 ± 34.3 μm2, range = 11.8–166.8 μm2). The present study suggests that Brn-3a may normally suppress TH expression in many small DRG neurons but activate TH expression in large DRG neurons.