ACTIVATION OF RAF-1 BY 14-3-3-PROTEINS
ACTIVATION OF RAF-1 BY 14-3-3-PROTEINS
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DOI：
10.1038/371612a0
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发表时间：
1994-10-13
期刊：
影响因子：
64.8
通讯作者：
WILLIAMS, LT
中科院分区：
文献类型：
--
作者：
FANTL, WJ;MUSLIN, AJ;WILLIAMS, LT
Raf-1蛋白是有丝分裂和分化的关键介质，与p21（ras）相关（参考文献1-3）。然而，Raf-1的丝氨酸/苏氨酸激酶活性的调节仍不清楚（4-13）。使用酵母双杂交系统（8，14 -16），我们鉴定了两种与Raf-1的氨基末端区域相互作用的结构相关蛋白。这些蛋白质14-3-3 zeta（PLA（2））和14-3-3 beta（HS 1）是14-3-3蛋白质家族的成员（17-23）。14-3-3蛋白在非洲爪蟾卵母细胞中的表达增强Raf-1活性并促进Raf-1依赖的卵母细胞成熟。Raf-1的显性负突变体阻断了14-3-3蛋白的作用。
THE protein Raf-1, a key mediator of mitogenesis and differentiation, associates with p21(ras) (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood(4-13). Using the yeast two-hybrid systems(8,14-16), we identified two structurally related proteins that interact with the aminoterminal region of Raf-1. These proteins, 14-3-3 zeta (PLA(2)) and 14-3-3 beta (HS1), are members of the 14-3-3 family of proteins(17-23). Expression of 14-3-3 proteins in Xenopus oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.