Determination of δ13C and δ15N and trophic fractionation in jellyfish: implications for food web ecology
Determination of δ13C and δ15N and trophic fractionation in jellyfish: implications for food web ecology
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水母 δ13C 和 δ15N 的测定以及营养分馏：对食物网生态学的影响
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
W. Graham
中科院分区：
文献类型：
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作者：
I. D’Ambra;R. Carmichael;W. Graham
稳定同位素分析（SIA）在水母中的应用允许定义的营养模式，不能单独使用肠道内容物分析检测，但分析协议需要标准化，以避免在解释同位素数据的偏见。本文测定了墨西哥湾北方（30°00′N，89°00′W-30°24′N，88°00′W）奥雷利亚属（Aurelia sp.）的δ 13 C和δ 15 N，以确定身体各部分和全身稳定同位素组成的差异、脂质提取对组织中δ 13 C的影响以及水母到猎物的分馏值。钟螺和整个奥雷利亚种的同位素组成没有差异。去除脂质后δ 13 C值增加，表明需要进行校正。为了帮助未来的分析，我们从水母样本的经验数据中推导出一个校正方程。实验室饲养实验表明，水母的δ 13 C和δ 15 N分别比饲料增加了+4 ‰和+0.1 ‰。这些结果表明，通常适用于其他物种的协议可能是不准确的定义奥雷利亚sp.营养生态。因为奥雷利亚属。在海洋生态系统中普遍存在，通过使用SIA准确定义其营养作用对了解全球海洋食物网具有重要意义。
Application of stable isotope analysis (SIA) in jellyfish allows definition of trophic patterns not detectable using gut content analysis alone, but analytical protocols require standardization to avoid bias in interpreting isotopic data. We determined δ13C and δ15N in Aurelia sp. from the northern Gulf of Mexico (30°00′N, 89°00′W–30°24′N, 88°00′W) to define differences in stable isotope composition between body parts and whole body, the effect of lipid extraction on δ13C in tissues, and fractionation values from medusa to prey. The isotopic composition of bell and whole Aurelia sp. was not different. The increase in δ13C values after lipid removal suggested a correction is needed. To aid future analyses, we derived a correction equation from empirical data for jellyfish samples. Laboratory feeding experiments indicated medusae increased +4 ‰ in δ13C and +0.1 ‰ in δ15N compared to their diet. These results suggest protocols commonly applied for other species may be inaccurate to define Aurelia sp. trophic ecology. Because Aurelia spp. are commonly found in marine ecosystems, accurately defining their trophic role by use of SIA has implications for understanding marine food webs worldwide.