Immunolocalization of CC10 in Clara cells in mouse and human lung.
Immunolocalization of CC10 in Clara cells in mouse and human lung.
复制标题
CC10 在小鼠和人肺 Clara 细胞中的免疫定位。
DOI：
10.1007/s004180100251
复制
发表时间：
2001
影响因子：
2.3
通讯作者：
deMello,DE
中科院分区：
文献类型：
--
作者：
Ryerse,JS;Hoffmann,JW;Mahmoud,S;Nagel,BA;deMello,DE
两种抗血清，表示为R41和R42，是针对来自鼠Clara细胞特异性蛋白CC 10的合成肽产生的，一种抗血清，表示为R40，是针对人重组子宫珠蛋白（鼠CC 10的人同源物）产生的。使用肽柱制备纯化的抗原特异性抗血清，表示为R40 AP、R41 AP和R42 AP。纯化的抗血清的特点是斑点印迹，免疫组织化学，免疫印迹。小鼠肺的免疫组织化学显示，所有三种抗血清均特异性标记远端细支气管中的Clara细胞。在人肺中，抗子宫珠蛋白抗血清特异性标记Clara细胞，而抗小鼠肽抗血清具有弱交叉反应性和较高的背景染色。电子显微镜显示免疫金标记的CC 10颗粒在克拉拉细胞的小鼠肺与所有的抗血清。所有的抗血清也标记了5-kDa的蛋白免疫印迹的小鼠肺匀浆。远端细支气管周围肺泡气隙的表面上皮细胞呈CC 10阳性，表明Clara细胞远端肺实质中CC 10的功能活性。正常人肺和来自表面活性蛋白B缺乏、支气管肺炎和特发性肺泡蛋白沉积症患者的肺切片的R40 AP免疫组织化学染色说明了抗人CC 10抗体用于诊断病理学的效用。
Two antisera, denoted R41 and R42, were raised against a synthetic peptide from the murine Clara cell-specific protein CC10, and one antiserum, denoted R40, was raised against human recombinant uteroglobin, the human homolog of murine CC10. Purified antigen-specific antisera, denoted R40AP, R41AP, and R42AP were prepared using peptide columns. The purified antisera were characterized by dot blots, immunohistochemistry, and immunoblots. Immunohistochemistry of mouse lung showed specific labeling of Clara cells in distal bronchioles by all three antisera. In human lung, the anti-uteroglobin antiserum specifically labeled Clara cells, while the anti-mouse peptide antisera had weak crossreactivity and higher background staining. Electron microscopy revealed immunogold labeling of CC10 granules in Clara cells of mouse lung with all antisera. All antisera also labeled a 5-kDa protein on immunoblots of mouse lung homogenates. The surface epithelium of the alveolar air spaces around the distal bronchioles were CC10 positive suggesting a functional activity for CC10 in the lung parenchyma distal to Clara cells. R40AP immunohistochemical staining of sections of normal human lungs and lungs from patients with surfactant protein B deficiency, bronchopneumonia, and idiopathic alveolar proteinosis illustrate the utility of the anti-human CC10 antibody for diagnostic pathology.