Autophagy induction upon reactive oxygen species in Cd-stressed Arabidopsis thaliana
Autophagy induction upon reactive oxygen species in Cd-stressed Arabidopsis thaliana
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DOI：
10.1117/12.841394
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发表时间：
2010-02
期刊：
影响因子：
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通讯作者：
Weina Zhang;Wenli Chen
中科院分区：
文献类型：
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作者：
Weina Zhang;Wenli Chen
自噬是一种蛋白质降解过程，其中细胞在受到环境胁迫条件或在某些发育阶段期间回收细胞质内容物。在诱导自噬后，在细胞质组分周围形成双膜自噬体，并将它们递送到液泡进行降解。在植物中，自噬先前已被证明在非生物胁迫包括氧化胁迫期间被诱导。镉作为一种毒性重金属，可导致活性氧（ROS）的产生。在本文中，我们证明，ROS有助于诱导自噬镉胁迫拟南芥。抗坏血酸（阿萨，抗氧化分子）和过氧化氢酶（CAT，H2 O2-特异性清除剂）预孵育可降低ROS的产生和自溶体样结构的数量。总之，我们的研究结果表明，氧化条件是必不可少的自噬，与阿萨和CAT处理废除自噬体的形成，和ROS可能作为信号分子诱导自噬在非生物胁迫。
Autophagy is a protein degradation process in which cells recycle cytoplasmic contents when subjected to environmental stress conditions or during certain stages of development. Upon the induction of autophagy, a double membrane autophagosome forms around cytoplasmic components and delivers them to the vacuole for degradation. In plants, autophagy has been shown previously to be induced during abiotic stresses including oxidative stress. Cd, as a toxicity heavy metal, resulted in the production of reactive oxygen species (ROS). In this paper, we demonstrated that ROS contributed to the induction of autophagy in Cd-stressed Arabidopsis thaliana. However, pre-incubation with ascorbic acid (AsA, antioxidant molecule) and catalase (CAT, a H2O2-specific scavenger) decreased the ROS production and the number of autolysosomal-like structures. Together our results indicated that the oxidative condition was essential for autophagy, as treatment with AsA and CAT abolished the formation of autophagosomes, and ROS may function as signal molecules to induce autophagy in abiotic stress.