Expression of the Tgfß2 Gene During Chick Embryogenesis
Expression of the Tgfß2 Gene During Chick Embryogenesis
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DOI：
10.1002/ar.22400
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发表时间：
2012-02-01
影响因子：
2
通讯作者：
Nakajima, Yuji
中科院分区：
文献类型：
--
作者：
Yamagishi, Toshiyuki;Ando, Katsumi;Nakajima, Yuji
我们使用原位杂交对鸡胚胎发生过程中从第6阶段到第30阶段转化生长因子（TGF）β 2的表达进行了全面分析（Hamburger和汉密尔顿，J Morphol 1951;88：4992）。在心脏发生过程中，TGF β 2在瓣膜间隔内膜垫组织的内皮/间充质细胞和心外膜中表达，直到胚胎发生结束。在大动脉形成过程中，TGF β 2定位于平滑肌祖细胞，而不是血管内皮细胞。在肢体发育过程中，TGF β 2在第16阶段在假定肢体区域的间充质细胞中表达，此后它定位于骨骼肌祖细胞中。此外，在咽弓的间充质细胞中观察到强烈的TGF β 2表达。TGF β 2 mRNA也在其他中胚层来源的组织中检测到，如发育中的骨和胸膜。在外胚层发育过程中，TGF β 2在神经管、透镜、视神经和耳泡的底板中表达。此外，TGF β 2在发育中的肠上皮中表达。我们的研究结果表明，TGF β 2起着重要的作用，不仅在上皮间质的相互作用，而且在细胞分化和迁移和细胞死亡在鸡胚胎发生。我们还发现鸡和小鼠TGF β 2 RNA在胚胎发生过程中表现出非常相似的表达模式。鸡胚可以作为一个有用的模型，以增加我们对TGF β 2在器官发生过程中的细胞相互作用，细胞分化和增殖中的作用的理解。Anat Rec，2012. (C)2011 Wiley Periodicals，Inc.
We performed a comprehensive analysis of the expression of transforming growth factor (TGF) beta 2 during chick embryogenesis from stage 6 to 30 (Hamburger and Hamilton, J Morphol 1951;88:4992) using in situ hybridization. During cardiogenesis, Tgf beta 2 was expressed in the endothelial/mesenchymal cells of the valvulo-septal endocardial cushion tissue and in the epicardium until the end of embryogenesis. During the formation of major arteries, Tgf beta 2 was localized in smooth muscle progenitors but not in the vascular endothelium. During limb development, Tgf beta 2 was expressed in the mesenchymal cells in the presumptive limb regions at stage 16, and thereafter it was localized in the skeletal muscle progenitors. In addition, strong Tgf beta 2 expression was seen in the mesenchymal cells in the pharyngeal arches. Tgf beta 2 mRNA was also detected in other mesoderm-derived tissues, such as the developing bone and pleura. During ectoderm development, Tgf beta 2 was expressed in the floor plate of the neural tube, lens, optic nerve, and otic vesicle. In addition, Tgf beta 2 was expressed in the developing gut epithelium. Our results suggest that TGF beta 2 plays an important role not only in epithelial-mesenchymal interactions but also in cell differentiation and migration and cell death during chick embryogenesis. We also found that chick and mouse Tgf beta 2 RNA show very similar patterns of expression during embryogenesis. Chick embryos can serve as a useful model to increase our understanding in the roles of TGF beta 2 in cellcell interactions, cell differentiation, and proliferation during organogenesis. Anat Rec, 2012. (C) 2011 Wiley Periodicals, Inc.