Diagnostic value of HCMV pp65 antigen detection by FCA for symptomatic and asymptomatic infection: compared to quantification of HCMV DNA and detection of IgM antibody in infants
Diagnostic value of HCMV pp65 antigen detection by FCA for symptomatic and asymptomatic infection: compared to quantification of HCMV DNA and detection of IgM antibody in infants
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FCA检测HCMV pp65抗原对有症状和无症状感染的诊断价值：与婴儿HCMV DNA定量和IgM抗体检测相比
DOI：
10.1007/s00430-009-0112-y
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发表时间：
2009-03
影响因子：
5.4
通讯作者：
Liu, Qing
中科院分区：
文献类型：
--
作者：
Ji, Yaohua;Mao, Zhiqin;Sun, Zhengrong;Ruan, Qiang;Qi, Ying;Wang, Jidong;He, Rong;Ma, Yanping;Ceng, Xiangshi;Liu, Qing
人巨细胞病毒（HCMV）可引起婴儿有症状或无症状感染。对126例婴儿进行了婴儿期疾病的临床评估。其中80例根据体格、仪器和实验室检查结果被归类为有症状感染，5例经随访证实后来发展为HCMV疾病，其他41例婴儿被归类为无症状感染。用定量聚合酶链反应检测所有有症状的婴儿尿标本中的HCMV DNA均为阳性。用荧光免疫法（CLIA）检测85例有症状患儿中62例HCMV-IgM抗体阳性，而无症状患儿均为阴性。85例有症状患儿中77例用流式细胞术（FCA）检测HCMV pp 65抗原阳性，无症状患儿中无一例HCMV pp 65抗原阳性。HCMVpp 65抗原检测与临床症状的符合率高于HCMVDNA和HCMV-IgM抗体检测。HCMV pp 65抗原检测诊断HCMV感染的敏感性为90.6%，特异性为100%，阳性预后值为100%，阴性预后值为83.7%。结论：FCA检测pp 65抗原对HCMV感染的诊断比检测HCMV-IgM抗体更敏感，在区分婴儿有症状和无症状HCMV感染方面优于HCMV DNA定量。
Human cytomegalovirus (HCMV) can cause symptomatic or asymptomatic infection in infants. One hundred and twenty-six infants were assessed clinically for disease in infantile period. Eighty of them were classified as symptomatic infection on the basis of physical, instrumental, and laboratory findings, 5 were demonstrated by following up to have later developed HCMV disease, and the other 41 infants were classified as asymptomatic infection. HCMV DNA was positive in all urine samples of the symptomatic infants detected by quantitative polymerase chain reaction. HCMV-IgM antibody detected by chemiluminescent immunoassay (CLIA) was positive in 62 of the 85 symptomatic infants, but was negative in all of the samples of asymptomatic infants. HCMV pp65 antigen detected by flow cytometry assay (FCA) was positive in 77 of the 85 symptomatic infants and in none of the asymptomatic infants. The coincidence to symptom of HCMV pp65 antigen detection was higher than those of HCMV DNA and HCMV-IgM antibody detection. The sensitivity, specificity, positive prognostic value and the negative prognostic value of HCMV pp65 antigen detection for diagnosis of HCMV infection was 90.6, 100, 100 and 83.7%, respectively. We concluded that detection of pp65 antigen by FCA is more sensitive for diagnosis of HCMV infection than detection of HCMV-IgM antibody and is better than HCMV DNA quantification for distinguishing the symptomatic and asymptomatic HCMV infection in infants.
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影响因子：
3.6
作者：
BOPPANA, SB;PASS, RF;ALFORD, CA
通讯作者：
ALFORD, CA
影响因子：
4.5
作者：
Cannon MJ;Davis KF
通讯作者：
Davis KF
影响因子：
9.4
作者：
K. St. George;M. J. Boyd;S. Lipson;D. Ferguson;G. F. Cartmell;L. Falk;C. Rinaldo;M. Landry
通讯作者：
K. St. George;M. J. Boyd;S. Lipson;D. Ferguson;G. F. Cartmell;L. Falk;C. Rinaldo;M. Landry
DOI：
10.1002/cyto.990150304
发表时间：
1994-03
期刊：
Cytometry
影响因子：
--
作者：
J. Ploem‐Zaaijer;W. Mesker;G. Boland;W. Sloos;F. M. van de Rijke;M. Jiwa;A. Raap
通讯作者：
J. Ploem‐Zaaijer;W. Mesker;G. Boland;W. Sloos;F. M. van de Rijke;M. Jiwa;A. Raap
影响因子：
12.7
作者：
Mengelle, C;Pasquier, C;Izopet, J
通讯作者：
Izopet, J