Stability of anti-HIV antibodies in serum samples stored for two to eighteen years periods
Stability of anti-HIV antibodies in serum samples stored for two to eighteen years periods
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DOI：
10.5935/1676-2444.20140026
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发表时间：
2014-08-01
影响因子：
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通讯作者：
Ueda, Mirthes
中科院分区：
文献类型：
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作者：
Castejon, Marcia Jorge;Yamashiro, Rosemeire;Ueda, Mirthes
简介：抗体在血清诊断中发挥着重要作用，是检测和确认各种疾病最广泛使用的生物标志物。目的：研究抗人类免疫缺陷病毒（HIV）抗体反应性的重现性，评估血清样本在-20°C保存2至18年的稳定性。方法：收集 1988 年至 2004 年期间的血清用于常规抗 HIV 抗体诊断检测。本研究对储存在 -20°C 下的剩余样品进行了分析。通过酶联免疫吸附测定/酶免疫测定（ELISA/EIA）、间接免疫荧光测定（IFA）和用于检测抗HIV抗体的蛋白质印迹（WB）评估血清样品稳定性。对先前发现的结果（1988-2004 年）和 2006 年获得的结果进行了 Kappa 指数分析。结果：1988年至2004年期间，ELISA/EIA、IFA和WB结果的一致性程度分别为良好（k=0.80）、正常（k=0.35）和良好（k=0.63）。结论：对于HIV血清学检测，血清样本在ELISA/EIA中稳定18年，在IFA技术中稳定4年，然而，对于WB方法来说，不可能确定抗HIV抗体的稳定时间。
Introduction: The antibodies have an important role in the serodiagnosis, constituting the most widely used biomarkers to detect and confirm various diseases. Objective: To investigate the reproducibility of anti-human immunodeficiency virus (HIV) antibodies reactivity, to assess the stability of the sera samples stored at -20°C for two to eighteen years. Method: Sera were collected in the period 1988-2004 for routine anti-HIV antibodies diagnostic testing. The remaining samples stored at -20°C, were analyzed in this study. Serum sample stability was assessed by enzyme-linked immunosorbent assay/enzyme immunoassay (ELISA/EIA), indirect immunofluorescence assay (IFA), and Western blot (WB) for detecting anti-HIV antibodies. The previously found results (1988-2004) and those obtained in 2006 were subjected to Kappa index analysis. Result: In the period 1988-to 2004, the degree of concordance of the ELISA/EIA, IFA and WB results were considered, good (k=0.80), regular (k=0.35), and good (k=0.63), respectively. Conclusion: Regarding HIV serologic test, the serum samples were stable for 18 years in ELISA/EIA and for 4 years in IFA technique, however, for the WB methodology it was not possible to determine the time of stability of the anti-HIV antibodies.