Refractoriness of the Sheep Superior Vena Cava Myocardial Sleeve
Refractoriness of the Sheep Superior Vena Cava Myocardial Sleeve
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绵羊上腔静脉心肌袖的不应期
DOI：
10.3181/0803-rm-102
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发表时间：
2008-11
影响因子：
3.2
通讯作者：
Zhang, Yangyang
中科院分区：
文献类型：
--
作者：
Zhao, Cuimei;Chen, Yi-Han;Liu, Xingyuan;Liu, Yi;Ma, Jun;Xu, Jiahong;Yang, Ying;Li, Li;Wu, Xianzheng;Liang, Lei;Xiao, Jinjie;Chen, Huaizhi;Li, Jun;Qi, Jie;Peng, Luying;Zhang, Yangyang
上级腔静脉心肌袖中的心肌细胞具有不同的动作电位和离子电流，但这些细胞的不应性尚未见报道。采用标准的细胞内微电极技术，我们在绵羊上腔静脉中证明了心肌细胞的有效不应期（ERP），（114.7 ± 6.5ms）短于下腔静脉（IVC）右房游离壁（RAFW）（201.0 ± 6.0）ms，右心耳（RAA）（203.1 ± 5.8）ms（P < 0.05）。乙酰胆碱（一种毒蕈碱2型受体（M2 R）激动剂）可不均匀地缩短右心房心肌细胞的ERP。灌注15 μM乙酰胆碱后，上腔静脉ERP最短（上腔静脉、下腔静脉、右前前脑和右前前脑ERP分别为53.6 ± 2.7、98.9 ± 2.2、121.8 ± 6.0和109.7 ± 5.1ms; P < 0.05）。卡巴胆碱（1 μM）是另一种M2 R激动剂，产生与乙酰胆碱相似的作用。此外，我们使用甲氧曲明，一种M2 R阻断剂，4-DAMP，一种毒蕈碱3型受体（M3 R）阻断剂，和托吡卡胺，一种毒蕈碱4型受体（M4 R）阻断剂抑制乙酰胆碱诱导的SVC心肌细胞ERP缩短，发现甲氧曲明，4-DAMP和托吡卡胺的50%抑制浓度分别为5.91，45.72和80.34 nM。因此，我们得出结论，羊SVC心肌袖是一个独特的电生理区域的右心房最短的ERP在生理条件下和胆碱能激动剂刺激。M2 R可能在SVC心肌袖对副交感神经张力的反应中起主要作用。上腔静脉的不同不应期与起源于该区域的房颤之间的关系值得进一步研究。
The cardiomyocytes in the superior vena cava (SVC) myocardial sleeve have distinct action potentials and ionic current profiles, but the refractoriness of these cells has not been reported. Using standard intracellular microelectrode techniques, we demonstrated in sheep that the effective refractory period (ERP) of the cardiomyocytes in the SVC (114.7 ± 6.5 ms) is shorter than that in the inferior vena cava (IVC) (166.7 ± 6.2 ms), right atrial free wall (RAFW) (201.0 ± 6.0 ms) and right atrial appendage (RAA) (203.1 ± 5.8 ms) (P < 0.05). The right atrial cardiomyocyte ERP was heterogeneously shortened by acetylcholine, a muscarinic type 2 receptor (M2R) agonist. After perfusion with 15 μM acetylcholine, the shortest ERP occurred in the SVC (the ERP in the SVC, IVC, RAFW and RAA was 53.6 ± 2.7, 98.9 ± 2.2, 121.8 ± 6.0 and 109.7 ± 5.1 ms, respectively; P < 0.05). Carbachol (1 μM), another M2R agonist, produced a similar effect as acetylcholine. Furthermore, we used methoctramine, a M2R blocker, 4-DAMP, a muscarinic type 3 receptor (M3R) blocker, and tropicamide, a muscarinic type 4 receptor (M4R) blocker to inhibit the acetylcholine-induced ERP shortening of SVC cardiomyocytes, and found that the 50% inhibitory concentration for methoctramine, 4-DAMP and tropicamide was 5.91, 45.72 and 80.34 nM, respectively. Therefore, we conclude that the sheep SVC myocardial sleeve is a unique electrophysiological region of the right atrium with the shortest ERP both under physiological condition and under cholinergic agonist stimulation. M2R might play a major role in the response of the SVC myocardial sleeve to parasympathetic nerve tone. The association between the distinct refractoriness in SVC and atrial fibrillation originating from the region deserves further investigation.
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影响因子：
2.7
作者：
Bien-Hsien Huang;Mei-han Wu;H. Tsao;C. Tai;Kun-Tai Lee;Yenn-Jiang Lin;M. Hsieh;Shih-Huang Lee;Yi‐Jen Chen;J. Kuo;Shih‐Ann Chen
通讯作者：
Bien-Hsien Huang;Mei-han Wu;H. Tsao;C. Tai;Kun-Tai Lee;Yenn-Jiang Lin;M. Hsieh;Shih-Huang Lee;Yi‐Jen Chen;J. Kuo;Shih‐Ann Chen
DOI：
10.1023/b:jice.0000042351.26751.3f
发表时间：
2004-10
影响因子：
1.8
作者：
T. Yamane;S. Miyanaga;K. Inada;Seiichiro Matsuo;H. Miyazaki;T. Date;Kunihiko Abe;Ken‐ichi Sugimoto;S. Mochizuki
通讯作者：
T. Yamane;S. Miyanaga;K. Inada;Seiichiro Matsuo;H. Miyazaki;T. Date;Kunihiko Abe;Ken‐ichi Sugimoto;S. Mochizuki
影响因子：
20.1
作者：
Kneller, J;Zou, RQ;Nattel, S
通讯作者：
Nattel, S
影响因子：
2.7
作者：
D. Shah;Michel Häissaguerre;Pierre Jäis;J. Clémenty
通讯作者：
D. Shah;Michel Häissaguerre;Pierre Jäis;J. Clémenty
影响因子：
20.1
作者：
SMEETS, JLRM;ALLESSIE, MA;HOLLEN, J
通讯作者：
HOLLEN, J