The visual physiology of the wild type mouse determined with pattern VEPs
The visual physiology of the wild type mouse determined with pattern VEPs
复制标题
DOI：
10.1016/s0042-6989(99)00022-x
复制
发表时间：
1999-09-01
期刊：
影响因子：
1.8
通讯作者：
Maffei, L
中科院分区：
文献类型：
--
作者：
Porciatti, V;Pizzorusso, T;Maffei, L
遗传操作小鼠是研究视觉系统可塑性和退化/再生的重要工具。然而，仍然缺乏对野生型小鼠视觉表现基本特性的描述。为了表征野生型（C57 BL/6 J）小鼠的视觉生理学，我们记录了来自初级视觉皮层的视觉诱发电位（VEP）。与行为方法相比，VEP可能具有以下优点：可以在相同的动物中容易且同时地筛选视觉的不同方面，包括由于运动或学习缺陷而具有不良视觉行为的动物。从麻醉小鼠的双眼视皮层记录了对图案化视觉刺激的局部VEP反应。空间（视力，对比度阈值）和时间（时间功能，反应潜伏期，运动敏感性）方面的VEP进行了评价。小鼠VEP敏锐度为0.6 c/deg，这与行为视觉敏锐度相当。VEP峰值对比度阈值为5%（无行为数据可用）。视觉坐标和皮质放大因子的皮质表示对应于先前使用单细胞记录的那些。VEP的层分析表明，在视皮层的颗粒上层的偶极子源作为一个主要的反应发生器。VEP显示了双眼的贡献，尽管强烈偏向记录皮层对侧的眼睛。结果提供了一个全面的框架来表征各种转基因小鼠的视觉表型。(C)1999 Elsevier Science Ltd.保留所有权利。
Genetically manipulated mice are important tools for studies on plasticity and degeneration/regeneration in the visual system. However, a description of the basic properties of the visual performance of the wild type mouse is still lacking. To characterize the visual physiology of the wild type (C57BL/6J) mouse we recorded Visual Evoked Potentials (VEPs) from the primary visual cortex. As compared to behavioral methods, VEPs may have the advantage that different aspects of vision can be screened readily and simultaneously in the same animals, including those with poor visual behavior due to motor or learning deficits. Local VEP responses to patterned visual stimuli have been recorded from the binocular visual cortex of anesthetized mice. Spatial (visual acuity, contrast threshold) and temporal (temporal function, response latency, motion sensitivity) aspects of VEPs were evaluated. The mouse VEP acuity was 0.6 c/deg, which is comparable to the behavioral visual acuity. The VEP peak contrast threshold was 5% (no behavioral data are available). Cortical representation of visual coordinates and cortical magnification factor corresponded to those previously reported using single cell recordings. Laminar analysis of VEPs indicated a dipole source in the supragranular layers of the visual cortex as a major response generator. VEPs showed contribution from both eyes, although biased strongly towards the eye contralateral to the recorded cortex. Results provide a comprehensive framework for characterizing visual phenotypes of a variety of transgenic mice. (C) 1999 Elsevier Science Ltd. All rights reserved.