Identification of genes in hepatocellular carcinoma induced by non-alcoholic fatty liver disease.
Identification of genes in hepatocellular carcinoma induced by non-alcoholic fatty liver disease.
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DOI：
10.3233/cbm-190169
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Yu C
中科院分区：
文献类型：
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作者：
Cai C;Song X;Yu C
肝细胞癌（HCC）是全世界死亡的主要原因。近年来，NAFLD诱发的HCC发病率呈快速增长趋势。 筛选NAFLD和HCC新的致病基因及相关通路，探讨NAFLD进展为HCC的发病机制。 基因表达微阵列（GSE74656、GSE62232）用于鉴定差异表达基因（DEG）。功能富集和通路富集分析表明这些DEG与细胞周期和细胞外外泌体相关，与NAFLD和HCC的发生密切相关。然后，我们使用相互作用基因检索搜索工具 (STRING) 建立蛋白质-蛋白质相互作用 (PPI) 网络，并在 Cytoscape 中将其可视化。并对hub基因进行总体生存（OS）分析和TCGA中的基因表达验证。 鉴定出七个中心基因，包括 CDK1、HSP90AA1、MAD2L1、PRKCD、ITGB3BP、CEP192 和 RHOB。最后，我们通过体外实时定量PCR验证了ITGB3BP和CEP192的表达水平。 目前的研究表明，NAFLD 发展为 HCC 的过程中可能存在 DEG，尤其是新基因 CEP192。上述结果还需要进一步严格的实验来验证。
Hepatocellular carcinoma (HCC) is the leading cause of mortality worldwide. In recent years, the incidence of HCC induced by NAFLD is growing rapidly. To screen for new pathogenic genes and related pathways both in NAFLD and HCC, and to explore the pathogenesis of progression from NAFLD to HCC. Gene expression microarrays (GSE74656, GSE62232) were used for identifying differentially expressed genes (DEGs). Functional enrichment and pathway enrichment analyses indicated that these DEGs were related to cell cycle and extracellular exosome, which were closely related to NAFLD and HCC development. We then used the Search Tool for the Retrieval of Interacting Genes (STRING) to establish the protein-protein interaction (PPI) network and visualized them in Cytoscape. And the overall survival (OS) analysis and gene expression validation in TCGA of hub genes was performed. Seven hub genes, including CDK1, HSP90AA1, MAD2L1, PRKCD, ITGB3BP, CEP192, and RHOB were identified. Finally, we verified the expression level of ITGB3BP and CEP192 by quantitative real-time PCR in vitro. The present study implied possible DEGs, especially the new gene CEP192, in the progression of NAFLD developing to HCC. Further rigorous experiments are required to verify the above results.