K(ATP) channels mediate the beneficial effects of chronic ethanol ingestion.
K(ATP) channels mediate the beneficial effects of chronic ethanol ingestion.
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K(ATP) 通道介导慢性乙醇摄入的有益作用。
DOI：
10.1152/ajpheart.2000.279.5.h2574
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Warltier,DC
中科院分区：
文献类型：
--
作者：
Pagel,PS;Toller,WG;Gross,ER;Gare,M;Kersten,JR;Warltier,DC
长期摄入低剂量乙醇通过激活腺苷受体和蛋白激酶C来保护心肌免受缺血损伤。我们验证了ATP依赖的钾通道(KATP)介导这些有益作用的假设。用乙醇(1.5g/kg)或水与干粮混合灌胃，每日2次，共12wk。急性血流动力学检测后，犬静脉注射生理盐水或格列本脲(0.1 mg/kg)，冠状动脉阻断60min，再灌注3h。心肌梗死面积(氯化三苯基四氮唑染色)显著缩小(P<；0.05)，与对照组(25±2%)相比，乙醇处理组心肌梗死面积减少到左心室危险面积的14±1%。格列本脲对心肌梗死面积无明显影响(25±3%)，但可阻断乙醇的保护作用(28±3%)。不同组间的血流动力学或冠脉侧支血流量(通过放射性微球)没有差异。结果表明，KATP通道介导了慢性饮酒的保护作用。
Chronic ingestion of low doses of ethanol protects the myocardium from ischemic injury by activating adenosine receptors and protein kinase C. We tested the hypothesis that ATP-dependent potassium (KATP) channels mediate these beneficial effects. Dogs were fed with ethanol (1.5 g/kg) or water mixed with dry food twice per day for 12 wk. After they were acutely instrumented for measurement of hemodynamics, dogs received saline (vehicle) or glyburide (0.1 mg/kg iv) and were subjected to 60 min of coronary artery occlusion followed by 3 h of reperfusion. Infarct size (through triphenyltetrazolium chloride staining) was significantly (P< 0.05) reduced to 14 ± 1% of the left ventricular area at risk in ethanol-pretreated dogs compared with controls (25 ± 2%). Glyburide alone did not affect infarct size (25 ± 3%) but abolished the protective effects of ethanol pretreatment (28 ± 3%). No differences in hemodynamics or coronary collateral blood flow (through radioactive microspheres) were observed among groups. The results indicate that KATPchannels mediate the protective effects of chronic consumption of ethanol.