Hydrogen peroxide detoxification in the midgut of the blood-sucking insect, Rhodnius prolixus
Hydrogen peroxide detoxification in the midgut of the blood-sucking insect, Rhodnius prolixus
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DOI：
10.1002/arch.1058
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发表时间：
2001-10-01
影响因子：
2.2
通讯作者：
Oliveira, PL
中科院分区：
文献类型：
--
作者：
Paes, MC;Oliveira, MB;Oliveira, PL
在这里，我们调查过氧化氢的生产和解毒的吸血半翅目，Rhodnius prolixus。超氧化物歧化酶（SOD）催化超氧自由基（O-2（-））歧化。该反应产生过氧化氢，其被抗氧化酶如过氧化氢酶（CAT）清除。SOD和CAT的活性被发现在所有的组织研究，最高的中肠。注射3-氨基-1，2，4-三唑（AT）可剂量依赖性地抑制CAT活性。用AT处理的昆虫表现出H2 O2水平的两倍增加。注射DL-丁硫氨酸-[S，R]-亚砜亚胺（BSO），谷胱甘肽合成的抑制剂，也导致过氧化氢的四倍增加，以及CAT活性的刺激。AT和BSO同时给药对中肠H2 O2含量有协同作用。综上所述，我们的研究结果表明，CAT和谷胱甘肽依赖的机制合作，以控制过氧化氢浓度在中肠细胞，并防止羟基自由基的产生在这个组织中的芬顿反应。(C)2001 Wiley-Liss，Inc.
Here we investigated H2O2 production and detoxification in the hematophagous hemiptera, Rhodnius prolixus. Superoxide dismutase (SOD) catalyzes the dismutation of superoxide radical (O-2(-)). This reaction produces hydrogen peroxide, which is scavenged by antioxidant enzymes such as catalase (CAT). SOD and CAT activities were found in all tissues studied, being highest in the midgut. CAT was dose-dependently inhibited in vivo by injections of 3-amino-1,2,4-triazole (AT). Insects treated with AT showed a twofold increase in H2O2 levels. Injection of DL-buthionine-[S, R]-sulfoximine (BSO), an inhibitor of glutathione synthesis, also resulted in a fourfold increase in H2O2, together with stimulation of CAT activity. Simultaneous administration of both AT and BSO had a synergistic effect on midgut H2O2 content. Taken all together, our results suggest that CAT and glutathione-dependent mechanisms cooperate to control H2O2 concentration in the midgut cell and prevent hydroxyl radical generation by Fenton reaction in this tissue. (C) 2001 Wiley-Liss, Inc.