IMPROVED COLOR REAGENT FOR DETERMINATION OF BLOOD-GLUCOSE BY OXIDASE SYSTEM
IMPROVED COLOR REAGENT FOR DETERMINATION OF BLOOD-GLUCOSE BY OXIDASE SYSTEM
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DOI：
10.1039/an9729700142
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发表时间：
1972-01-01
期刊：
影响因子：
4.2
通讯作者：
TRINDER, P
中科院分区：
文献类型：
--
作者：
BARHAM, D;TRINDER, P
本文提出了一种以4-氨基比林为成色剂，用磺化2，4-二氯苯酚作显色剂，测定葡萄糖氧化酶催化葡萄糖生成过氧化氢的方法，其灵敏度为：在10 mm比色皿中，20 μg葡萄糖在4 ml终体积中，于515 nm处的光密度为0.61，对应于22000的分子吸收。
A method involving the use of 4-aminophenazone as a colour coupler with sulphonated 2,4-dichlorophenol is described for determining the hydrogen peroxide produced from glucose with glucose oxidase.The sensitivity of the method is such that 20 µg of glucose in a final volume of 4 ml give an optical density of 0·61 at 515 nm with 10-mm cells, corresponding to a molecular absorption of 22 000.