Glutamate concentrations in human brain using single voxel proton magnetic resonance spectroscopy at 3 Tesla
Glutamate concentrations in human brain using single voxel proton magnetic resonance spectroscopy at 3 Tesla
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DOI：
10.1016/j.neuroimage.2003.11.014
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发表时间：
2004-04-01
期刊：
影响因子：
5.7
通讯作者：
Rinneberg, H
中科院分区：
文献类型：
--
作者：
Schubert, F;Gallinat, J;Rinneberg, H
建立了一种基于3 T磁共振波谱（MRS）的人脑谷氨酸（Glu）定量测定方法，并通过对40例健康志愿者大脑前扣带回皮质（acc）和左侧海马（he）的体素重复分析进行了验证。在80 ins的最佳回波时间，谷氨酸的C4共振似乎很好地解决和分离的主要干扰物，即谷氨酰胺和N-乙酰天冬氨酸。作为一种补充方法，采用了用于Glu的多量子相干滤波器序列。对于Glu和主要MRS可见代谢物的定量以及谷氨酰胺水平的估计，通过涉及从体模光谱获得的先验知识的时域-频域方法对两种类型的体内光谱进行分析。使用PRESS，Glu浓度的变异系数（CV）约为10%。当通过使用自旋分割获得的单个脑脊液组分校正浓度时，CV倾向于增加，两个MRS会话的相关系数倾向于降低，表明这种类型的校正增加了数据的不确定性。两个体素中Glu浓度有显著性差异（ace为11.6mmol/l，hc为10.9mmol/l，P = 0.023），且随年龄增长而降低（P < 0.04）。这些浓度与使用量子相干滤波器方法确定的浓度一致，尽管后者的不确定性限制了可靠的分析。(C)2004年爱思唯尔公司All rights reserved.
A method for quantitative determination of the glutamate (Glu) concentration in human brain using PRESS-based single voxel MR spectroscopy (MRS) at 3 T has been developed and validated by repeatedly analyzing voxels comprising the anterior cingulate cortex (acc) and the left hippocampus (he) in 40 healthy volunteer brains. At an optimum echo time of 80 ins, the C4 resonance of Glu appears well resolved and separated from major interferents, that is, glutamine and N-acetylaspartate. As a complementary method, a multiple quantum coherence filter sequence for Glu was employed. For quantification of Glu and the principal MRS visible metabolites as well as for an estimate of the glutamine level, analysis of both types of in vivo spectra was carried out by a time domain-frequency domain method involving prior knowledge obtained from phantom spectra. Using PRESS, coefficients of variation (CV) for Glu concentration were of the order of 10%. When the concentrations were corrected by individual cerebrospinal fluid fractions obtained by segmentation using spin, CVs tended to increase and the correlation coefficients for the two MRS sessions tended to decrease, indicating that this type of correction adds uncertainty to the data. The concentrations of Glu in the two voxels studied were found to be significantly different (11.6 mmol/l in ace, 10.9 mmol/l in hc, P = 0.023) and decrease with age (P < 0.04). These concentrations agreed well with those determined using the quantum coherence filter method although the uncertainty of the latter limits reliable analysis. (C) 2004 Elsevier Inc. All rights reserved.