THE B-MYCOIDES-N HOST-VIRUS SYSTEM .2. INTERRELATION OF PHAGE GROWTH, BACTERIAL MULTIPLICATION, AND LYSIS IN INFECTIONS OF THE INDICATOR STRAIN OF B-MYCOIDES-N WITH PHAGE-N IN NUTRIENT BROTH

THE B-MYCOIDES-N HOST-VIRUS SYSTEM .2. INTERRELATION OF PHAGE GROWTH, BACTERIAL MULTIPLICATION, AND LYSIS IN INFECTIONS OF THE INDICATOR STRAIN OF B-MYCOIDES-N WITH PHAGE-N IN NUTRIENT BROTH
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DOI:
10.1085/jgp.36.1.111
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发表时间:
1952-01-01
影响因子:
3.8
通讯作者:
KRUEGER, AP
KRUEGER, AP
中科院分区:
医学2区
文献类型:
--
作者:
BAER, BS;KRUEGER, AP

文献摘要

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在30至32[degree]C的营养肉汤中,病毒在B的产蛋期细胞中的生长周期(吸附后)。蕈状[芽孢杆菌] N包括细胞内增殖期,范围为0.8至1.3小时,随后是游离噬菌体滴度的急剧上升,然后是细胞外噬菌体含量的缓慢上升或平稳期。在30[degree]C下每个感染细胞的病毒产量,以detd.用改良的Burnett稀释法测定,约为76个噬菌斑形成颗粒。在潜伏期,多重感染细胞的数量没有变化。与噬菌体释放同时,发生不完全清除。未裂解的剩余细胞繁殖,浊度再次升高。这些幸存者和他们的后代是溶原性的。
In nutrient broth at 30 to 32[degree]C, the cycle of virus growth (following adsorption) in lay phase cells of B. [Bacillus] mycoides N included a period of intracellular multiplication, ranging from 0.8 to 1.3 hrs., succeeded by a sharp rise in the free phage titer and then by a slower rise or a plateau in the extracellular phage content. The yield of virus per infected cell at 30[degree]C, as detd. by a modified Burnett dilution technic, was about 76 plaque-forming particles. During the latent period, multiply infected cells showed no change in numbers. Coinciding with phage release, incomplete clearing occurred. The unlysed, remaining cells multiplied and the turbidity rose again. These survivors and their progeny were lysogenic.