Expression of TLR2 and TLR4 in murine small intestine during postnatal development
Expression of TLR2 and TLR4 in murine small intestine during postnatal development
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DOI：
10.1080/09168451.2016.1254534
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发表时间：
2017-02-01
影响因子：
1.6
通讯作者：
Tsukahara, Takamitsu
中科院分区：
文献类型：
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作者：
Inoue, Ryo;Yajima, Takaji;Tsukahara, Takamitsu
肠道微生物群在宿主免疫中发挥的重要作用部分是通过 Toll 样受体 (TLR) 介导的。我们评估了小鼠小肠中 TLR2 和 TLR4 表达的出生后变化，并评估了肠道微生物群对表达的影响。小鼠小肠中 TLR2 和 TLR4 的表达在发育过程中呈高度动态变化。这些变化在哺乳期尤其显着，在哺乳中期检测到最大 mRNA 水平。免疫组织化学和流式细胞术分析表明，TLR2 和 TLR4 表达的变化主要涉及上皮细胞。无菌小鼠在哺乳期TLR2/TLR4 mRNA和TLR2蛋白表现出微小变化。这项研究表明，小肠上皮细胞中 TLR2 和 TLR4 的出生后表达是动态的，并且取决于共生肠道微生物群的存在。
The important role played by the gut microbiota in host immunity is mediated, in part, through toll-like receptors (TLRs). We evaluated the postnatal changes in expression of TLR2 and TLR4 in the murine small intestine and assessed how expression is influenced by gut microbiota. The expression of TLR2 and TLR4 in the murine small intestine was highly dynamic during development. The changes were especially profound during the suckling period, with the maximal mRNA levels detected in the mid-suckling period. Immunohistochemical and flow-cytometric analyses indicated that the changes in TLR2 and TLR4 expression involve primarily epithelial cells. The germ-free mice showed minor changes in TLR2/TLR4 mRNA and TLR2 protein during the suckling period. This study demonstrated that the postnatal expression of TLR2 and TLR4 in small intestinal epithelial cells is dynamic and depends on the presence of commensal intestinal microbiota.