Selective interaction of JNK protein kinase isoforms with transcription factors
Selective interaction of JNK protein kinase isoforms with transcription factors
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DOI：
10.1002/j.1460-2075.1996.tb00636.x
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发表时间：
1996-06-03
期刊：
影响因子：
11.4
通讯作者：
Davis, RJ
中科院分区：
文献类型：
--
作者：
Gupta, S;Barrett, T;Davis, RJ
JNK蛋白激酶是MAP激酶组的一员，在苏氨酸和酪氨酸的双重磷酸化反应中被激活。通过分子克隆技术鉴定了人脑中10种JNK亚型。这些蛋白激酶对应于来自JNK1、JNK2和JNK3基因的选择性剪接异构体。利用转录因子ATF2、Elk-1和Jun家族成员作为底物，测量了这些JNK亚型的蛋白激酶活性。用白细胞介素-1 （IL-1）处理细胞引起JNK亚型的激活。这种激活被MAP激酶磷酸酶MKP-1的表达阻断。JNK亚型的结合活性比较表明，JNK蛋白与ATF2、Elk-1和Jun转录因子的相互作用存在差异。因此，JNK组的个体成员可以在体内选择性地靶向特定的转录因子。
The JNK protein kinase is a member of the MAP kinase group that is activated in response to dual phosphorylation on threonine and tyrosine. Ten JNK isoforms were identified in human brain by molecular cloning. These protein kinases correspond to alternatively spliced isoforms derived from the JNK1, JNK2 and JNK3 genes. The protein kinase activity of these JNK isoforms was measured using the transcription factors ATF2, Elk-1 and members of the Jun family as substrates. Treatment of cells with interleukin-1 (IL-1) caused activation of the JNK isoforms. This activation was blocked by expression of the MAP kinase phosphatase MKP-1. Comparison of the binding activity of the JNK isoforms demonstrated that the JNK proteins differ in their interaction with ATF2, Elk-1 and Jun transcription factors. Individual members of the JNK group may therefore selectively target specific transcription factors in vivo.