Morphological and histochemical characterization of the secretory epithelium in the canine lacrimal gland.
Morphological and histochemical characterization of the secretory epithelium in the canine lacrimal gland.
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DOI：
10.4081/ejh.2021.3320
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发表时间：
2021-11-02
期刊：
影响因子：
--
通讯作者：
Gomi H
中科院分区：
文献类型：
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作者：
Yasui T;Miyata K;Nakatsuka C;Tsukise A;Gomi H
在本研究中，分泌成分和囊泡转运蛋白在犬泪腺的表达进行了检查和形态计量学分析。分泌上皮由两种不同形态的分泌细胞组成。构成腺泡单位的分泌细胞（A型细胞）比构成管状单位的其他细胞类型（T型细胞）显示出更高水平的糖缀合物，包括β-GlcNAc。免疫印迹分析显示，抗微生物蛋白，如溶菌酶，乳铁蛋白和乳过氧化物酶，Rab蛋白（Rab 3d，Rab 27 a和Rab 27 b）和可溶性N-乙基马来酰亚胺敏感性融合蛋白附着蛋白受体（SNARE）蛋白（VAMP 2，VAMP 4，VAMP 8，突触融合蛋白-1，突触融合蛋白-4和突触融合蛋白-6），以不同的水平表达。我们用化学方法证明了溶菌酶、乳铁蛋白、Rab 27 a、Rab 27 b、VAMP 4、VAMP 8和syntaxin-6的表达模式因分泌细胞类型而异。此外，在T型细胞中，VAMP 4仅限于分泌颗粒的亚群，而VAMP 8在几乎所有的分泌颗粒中都被检测到。本研究显示了犬泪腺分泌上皮的形态学和组织化学特征。这些发现将有助于阐明这种腺体的物种特异性。
In the present study, the expression of secretory components and vesicular transport proteins in the canine lacrimal gland was examined and morphometric analysis was performed. The secretory epithelium consists of two types of secretory cells with different morphological features. The secretory cells constituting acinar units (type A cells) exhibited higher levels of glycoconjugates, including β-GlcNAc, than the other cell type constituting tubular units (type T cells). Immunoblot analysis revealed that antimicrobial proteins, such as lysozyme, lactoferrin and lactoperoxidase, Rab proteins (Rab3d, Rab27a and Rab27b) and soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins (VAMP2, VAMP4, VAMP8, syntaxin-1, syntaxin-4 and syntaxin-6), were expressed at various levels. We immunohistochemically demonstrated that the expression patterns of lysozyme, lactoferrin, Rab27a, Rab27b, VAMP4, VAMP8 and syntaxin-6 differed depending on the secretory cell type. Additionally, in type T cells, VAMP4 was confined to a subpopulation of secretory granules, while VAMP8 was detected in almost all of them. The present study displayed the morphological and histochemical characteristics of the secretory epithelium in the canine lacrimal gland. These findings will help elucidate the species-specific properties of this gland.