The identification of gonadotrophin surge inhibiting factor and its role in the regulation of pituitary gonadotrophin secretion.
The identification of gonadotrophin surge inhibiting factor and its role in the regulation of pituitary gonadotrophin secretion.
复制标题
促性腺激素激增抑制因子的鉴定及其在调节垂体促性腺激素分泌中的作用。
DOI：
10.1093/humrep/8.suppl_2.117
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Cheng,CY
中科院分区：
文献类型：
--
作者：
Danforth,DR;Cheng,CY
来自几个实验室的证据表明，大鼠、猪、猴和女性的卵巢产生一种非甾体因子，抑制或减弱周期中期促性腺激素激增。这种物质存在于卵泡液中，我们称之为促性腺激素峰抑制因子（GnSIF）。利用大鼠垂体细胞生物测定法，我们监测了GnSIF的活性，从猪卵泡液（PFF）纯化过程中。将短期培养的大鼠垂体细胞与GnSIF孵育48 h，清洗，然后暴露于促性腺激素释放激素（GnRH）加GnSIF 4 h。GnSIF活性定义为4 h内GnRH刺激的LH分泌抑制，而Bisabin活性表示为48 h内基础促卵泡激素（FSH）分泌抑制。通过肝素-琼脂糖凝胶、Q-琼脂糖凝胶、Mono-S、羟基磷灰石和凝胶渗透色谱步骤分级分离约51 μ l的PFF。这种分级分离去除了所有可检测的卵泡抑素和卵泡抑素免疫活性和生物活性。纯化的GnSIF抑制GnRH刺激的LH分泌，对基础FSH释放几乎没有影响。总之，我们已经获得了GnSIF的纯化制剂，其含有检测不到的促卵泡素和卵泡抑素。因此，GnSIF似乎与其他已知的调节生殖功能的性腺蛋白不同，并且可能与促性腺激素、激活素和卵泡抑素沿着参与垂体促性腺激素分泌的性腺调节。
Evidence from several laboratories suggests that the ovaries of rats, pigs, monkeys and women produce a non-steroidal factor which inhibits or attenuates the mid-cycle gonadotrophin surge. This substance is present in follicular fluid and we have called it gonadotrophin surge inhibiting factor (GnSIF). Utilizing a rat pituitary cell bioassay, we have monitored GnSIF activity during purification from porcine follicular fluid (PFF). Rat pituitary cells in short-term culture were incubated with GnSIF for 48 h, washed and then exposed to gonadotrophin releasing hormone (GnRH) plus GnSIF for 4 h. GnSIF activity was defined as suppression of GnRH-stimulated LH secretion over 4 h, and inhibin activity was expressed as suppression of basal follicle stimulating hormone (FSH) secretion over 48 h. Approximately 51 of PFF was fractionated through heparin–Sepharose, Q-Sepharose, Mono-S, hydroxylapatite, and gel permeation chromatography steps. This fractionation removes all detectable inhibin and follistatin immunoactivity and bioactivity. The purified GnSIF inhibits GnRH-stimulated LH secretion with little or no effect on basal FSH release. In summary, we have obtained a purified preparation of GnSIF which contains undetectable inhibin and follistatin. As such, GnSIF appears to be distinct from other known gonadal proteins regulating reproductive function, and may participate along with the inhibins, activins and foUistatins in the gonadal regulation of pituitary gonadotrophin secretion.