Migration of zebrafish primordial germ cells: A role for myosin contraction and cytoplasmic flow
Migration of zebrafish primordial germ cells: A role for myosin contraction and cytoplasmic flow
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DOI：
10.1016/j.devcel.2006.09.023
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发表时间：
2006-11-01
影响因子：
11.8
通讯作者：
Raz, Erez
中科院分区：
文献类型：
--
作者：
Blaser, Heiko;Reichman-Fried, Michal;Raz, Erez
在趋化因子SDF-1的作用下，调控细胞运动和定向迁移的分子和细胞机制在很大程度上是未知的。在这里，我们证明了斑马鱼原始生殖细胞的迁移是由SDF-1产生水泡样突起，由细胞质流供电。突起形成于游离钙水平较高的部位，在该处肌球蛋白收缩被激活。在这些部位，肌动蛋白-肌球蛋白皮质从质膜上分离，随后细胞质流入正在形成的大泡中。我们提出受体CXCR 4的极化激活导致游离钙的升高，这反过来激活了对更高水平的配体SDF-1做出反应的细胞部分中的肌球蛋白收缩。在细胞的特定区域中响应于SDF-1的新突起的偏置形成限定了细胞迁移的前沿和方向。
The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.