Efficacy and safety of spot heating and ultrasound irradiation on in vitro and in vivo thrombolysis models
Efficacy and safety of spot heating and ultrasound irradiation on in vitro and in vivo thrombolysis models
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DOI：
10.1177/0271678x221079127
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发表时间：
2022-02
影响因子：
6.3
通讯作者：
R. Morihara;T. Yamashita;Yosuke Osakada;Tian Feng;Xinran Hu;Y. Fukui;Koh Tadokoro;M. Takemoto
中科院分区：
文献类型：
--
作者：
R. Morihara;T. Yamashita;Yosuke Osakada;Tian Feng;Xinran Hu;Y. Fukui;Koh Tadokoro;M. Takemoto
虽然热或机械机制与溶栓结果之间的关系鲜为人知，但经颅超声溶栓的可行性已被证明。因此，本研究旨在通过体外和体内溶栓模型来揭示温度和超声的效果和安全性。将微管中的人工凝块在水浴中加热或超声辐照，确定凝块重量随温度升高和超声时间延长而减小。在体外血栓闭塞模型中，基于点状加热，血栓体积减少，血栓移动到远端，随后再通闭塞。在活体研究中，将大鼠的颈总动脉暴露在点状加热器或超声波下。未发现脑梗塞或脑血屏障破坏，但检测到颈动脉内皮细胞连接不完整和炎症反应。目前的点状加热和超声辐照模型对体外崩解凝块似乎是有效的，但体内模型的安全性并未得到数据的完全支持。然而，数据表明，较短的暴露时间可能比较长的暴露时间侵害性更小。
The feasibility of transcranial sonothrombolysis has been demonstrated, although little is known about the relationships between thermal or mechanical mechanisms and thrombolytic outcomes. Therefore, the present study aims to reveal the effect and safety of temperature and ultrasound through in vitro and in vivo thrombolysis models. Artificial clots in microtubes were heated in a water bath or sonicated by ultrasound irradiation, and then clots weight decrease with rising temperature and sonication time was confirmed. In the in vitro thrombotic occlusion model, based on spot heating, clot volume was reduced and clots moved to the distal side, followed by recanalization of the occlusion. In the in vivo study, the common carotid artery of rats was exposed to a spot heater or to sonication. No brain infarct or brain blood barrier disruption was shown, but endothelial junctional dysintegrity and an inflammatory response in the carotid artery were detected. The present spot heating and ultrasound irradiation models seem to be effective for disintegrating clots in vitro, but the safety of the in vivo model was not fully supported by the data. However, the data indicates that a shorter time exposure could be less invasive than a longer exposure.