Near-infrared-emissive polymersomes:: Self-assembled soft matter for in vivo optical imaging
Near-infrared-emissive polymersomes:: Self-assembled soft matter for in vivo optical imaging
复制标题
DOI：
10.1073/pnas.0409394102
复制
发表时间：
2005-02-22
影响因子：
11.1
通讯作者：
Therien, MJ
中科院分区：
文献类型：
--
作者：
Ghoroghchian, PP;Frail, PR;Therien, MJ
我们证明，合成软材料可以扩展天然囊泡的效用，从主要是亲水性水库功能胶体载体，促进大型水不溶性化合物的生物医学应用。近红外（NIR）发射聚合物囊泡（直径为50 nm至50 μ m的聚合物囊泡）通过两亲性二嵌段共聚物和共轭多（卟啉）基近红外荧光团（NIRF）的协同自组装而产生。当与天然囊泡磷脂相比，聚合物囊泡是唯一能够纳入和均匀分布的许多大型疏水NIRF专门在其层状膜。在这些隔离的隔间，长的聚合物链调节的平均荧光团荧光团间的空间分离，以及荧光团本地化的电子环境。基于卟啉的NIRF在聚合物体基质内表现出类似于在有机溶剂中为这些高发射偶极强度荧光团建立的那些物理性质，从而产生独特的发射囊泡。此外，从组件发出的总荧光产生足够强度的局部光学信号以穿透活体动物的致密肿瘤组织。因此，强大的近红外发射聚合物囊泡定义了一个软物质平台，具有特殊的潜力，以促进深部组织荧光成像的体内诊断和药物输送应用。
We demonstrate that synthetic soft materials can extend the utility of natural vesicles, from predominantly hydrophilic reservoirs to functional colloidal carriers that facilitate the biomedical application of large aqueous-insoluble compounds. Near-infrared (NIR)emissive polymersomes (50-nm- to 50-mum-diameter polymer vesicles) were generated through cooperative self assembly of amphiphilic diblock copolymers and conjugated multi(porphyrin)based NIR fluorophores (NIRFs). When compared with natural vesicles comprised of phospholipids, polymersomes were uniquely capable of incorporating and uniformly distributing numerous large hydrophobic NIRFs exclusively in their lamellar membranes. Within these sequestered compartments, long polymer chains regulate the mean fluorophore-fluorophore interspatial separation as well as the fluorophore-localized electronic environment. Porphyrin-based NIRFs manifest photophysical properties within the polymersomal matrix akin to those established for these high-emission dipole strength fluorophores in organic solvents, thereby yielding uniquely emissive vesicles. Furthermore, the total fluorescence emanating from the assemblies gives rise to a localized optical signal of sufficient intensity to penetrate through the dense tumor tissue of a live animal. Robust NIR-emissive polymersomes thus define a soft matter platform with exceptional potential to facilitate deep-tissue fluorescence-based imaging for in vivo diagnostic and drug-delivery applications.