Immobilizing live bacteria for AFM imaging of cellular processes
Immobilizing live bacteria for AFM imaging of cellular processes
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DOI：
10.1016/j.ultramic.2009.01.012
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发表时间：
2009-06-01
期刊：
影响因子：
2.2
通讯作者：
Hobbs, J. K.
中科院分区：
文献类型：
--
作者：
Kailas, L.;Ratcliffe, E. C.;Hobbs, J. K.
细菌物种金黄色葡萄球菌的球形细胞已被机械地捕获在光刻图案化的基板上，并使用原子力显微镜（AFM）在生长培养基下成像，以跟踪细胞过程。细胞不会受到干扰，因为表面没有化学连接。与将细胞捕获在多孔膜或软凝胶中相比，限制效应被最小化。S.金黄色葡萄球菌细胞被捕获在图案化的基底中时经历细胞分裂。在光刻图案化的衬底中的捕获提供了一种用于锚定细菌细胞的新方法，使得AFM针尖在成像期间不会将细胞推开，同时允许细菌继续进行细胞过程。(C)2009 Elsevier B.V. Ail版权所有。
Coccoid cells of the bacterial species Staphylococcus aureus have been mechanically trapped in lithographically patterned substrates and imaged under growth media using atomic force microscopy (AFM) in order to follow cellular processes. The cells are not perturbed as there is no chemical linkage to the surface. Confinement effects are minimized compared to trapping the cells in porous membranes or soft gels. S. aureus cells have been imaged undergoing cell division whilst trapped in the patterned substrates. Entrapment in lithographically patterned substrates provides a novel way for anchoring bacterial cells so that the AFM tip will not push the cells off during imaging, whilst allowing the bacteria to continue with cellular processes. (C) 2009 Elsevier B.V. Ail rights reserved.