ELEVATED TRANSFERRIN RECEPTOR CONTENT IN HUMAN PROSTATE-CANCER CELL-LINES ASSESSED INVITRO AND INVIVO
ELEVATED TRANSFERRIN RECEPTOR CONTENT IN HUMAN PROSTATE-CANCER CELL-LINES ASSESSED INVITRO AND INVIVO
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DOI：
10.1016/s0022-5347(17)39970-6
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发表时间：
1990-02-01
影响因子：
6.6
通讯作者：
GRAYHACK, JT
中科院分区：
文献类型：
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作者：
KEER, HN;KOZLOWSKI, JM;GRAYHACK, JT
用125I-转铁蛋白处理后，用Scatchard分析方法检测良性和恶性前列腺细胞的转铁蛋白受体(TFR)。已建立的人前列腺癌细胞系(PC-3和DU-145)以及具有生物侵袭性的变异体(PC-3 ASC和PC-3 DES)在体外评估时显示出显著的高亲和力TFR水平。相反，从人类良性前列腺增生症(BPH)标本中提取的培养基质细胞组分中，TFR的含量可以忽略不计。Scatchard分析还对体内来源的前列腺组织进行了分析：PC-3 ASC细胞皮下移植到裸鼠、裸鼠和新鲜BPH手术标本中所产生的肿瘤。这些组织被分离，它们的间质和上皮成分被分离。TFR仅在恶性和良性上皮细胞的上皮成分中表达。PC-3ASC肿瘤细胞的TFR水平与其体外表达水平相当，并且这些水平是BPH细胞的10倍。这些发现表明，升高的TFR可能是人类前列腺癌系统内转化表型的另一个有用的标志。
Transferrin receptors (TfR) were measured in benign and malignant prostatic cells by performing Scatchard analysis following the adminstration of 125I-transferrin. Established human prostate cancer cell lines (PC-3 and DU-145) as well as biologically aggressive variants (PC-3 ASC and PC-3 DES) were shown to possess significant levels of high affinity TfR when assessed in vitro. In contrast, TfR content was negligible in cultured stromal cell fractions derived from human benign prostatic hyperplasia (BPH) specimens. Scatchard analysis was also performed on in vivo derived prostatic tissues: tumors resulting from the subcutaneous xenografting of PC-3 ASC cells into athymic, nude mice and fresh BPH surgical specimens. These tissues were dissociated and their stromal and epithelial components separated. TfR were only detected in the epithelial component of both malignant and benign epithelial cells. PC-3 ASC tumor cells exhibited TfR levels comparable to their in vitro expression and these levels were 10-fold greater than in the BPH cells. These findings suggest that elevated TfRs may serve as another useful marker of transformed phenotype within human prostate tumor systems.