Peptide nucleic acid (PNA)/DNA hybrid duplexes: intercalation by an internally linked anthraquinone.
Peptide nucleic acid (PNA)/DNA hybrid duplexes: intercalation by an internally linked anthraquinone.
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肽核酸 (PNA)/DNA 杂合双链体：通过内部连接的蒽醌进行嵌入。
DOI：
10.1093/nar/26.3.715
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发表时间：
1998
影响因子：
14.9
通讯作者：
Schuster,GB
中科院分区：
文献类型：
--
作者：
Armitage,B;Koch,T;Frydenlund,H;Orum,H;Schuster,GB
肽核酸（PNA）通过在沃森-克里克碱基配对后形成互补双链体结构来模拟DNA和RNA。已知一组通过嵌入与DNA结合的报告化合物，但这些化合物不嵌入PNA/DNA杂合双链体中。杂交PNA双链体的分析需要开发探测其化学和物理性质的报告化合物。我们制备了一系列蒽醌（AQ）衍生物，连接到PNA寡聚体的内部位置。这些是第一个被掺入PNA的非核碱基官能团。所得PNA（AQ）缀合物与互补DNA寡聚物形成稳定的杂交体。我们发现，当AQ基团共价结合到PNA时，它们稳定了杂交双链体，并且至少部分地被插入。
Peptide nucleic acids (PNA) mimic DNA and RNA by forming complementary duplex structures following Watson-Crick base pairing. A set of reporter compounds that bind to DNA by intercalation are known, but these compounds do not intercalate in PNA/DNA hybrid duplexes. Analysis of the hybrid PNA duplexes requires development of reporter compounds that probe their chemical and physical properties. We prepared a series of anthraquinone (AQ) derivatives that are linked to internal positions of a PNA oligomer. These are the first non-nucleobase functional groups that have been incorporated into a PNA. The resulting PNA(AQ) conjugates form stable hybrids with complementary DNA oligomers. We find that when the AQ groups are covalently bound to PNA that they stabilize the hybrid duplex and are, at least partially, intercalated.
醌作为诱变剂、致癌剂和抗癌剂：介绍和概述。
DOI：
10.1080/15287398509530776
发表时间：
1985
期刊：
Journal of toxicology and environmental health
影响因子：
--
作者：
Smith,MT
通讯作者：
Smith,MT