High resolution colonoscopy in live mice
High resolution colonoscopy in live mice
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DOI：
10.1038/nprot.2006.446
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发表时间：
2006-01-01
期刊：
影响因子：
14.8
通讯作者：
Neurath, M. F.
中科院分区：
文献类型：
--
作者：
Becker, C.;Fantini, M. C.;Neurath, M. F.
对于医生来说，内窥镜检查是检查肠道炎性或肿瘤性变化的有效方法。在医学和免疫学研究中，建立肠道疾病的动物模型是研究粘膜免疫系统、结肠炎和肠道癌症发展的关键工具。此外，这些模型代表了测试新药的有效系统。在过去，为了分析结肠炎的活动和肿瘤的发展，必须杀死小鼠。以下方案描述了一种对活体小鼠进行高分辨率内窥镜监测的方法。患有结肠炎或结肠肿瘤的小鼠被麻醉，并用微型内窥镜进行检查。内窥镜通过肛门进入，然后用空气泵小心地给结肠充气。获得的内窥镜图像质量很高，可以对肿瘤和炎症进行监测和分级。此外，还可以进行结肠活检。该方案可在1小时内完成。
Endoscopy in humans is a powerful method for physicians to examine the gut for inflammatory or neoplastic changes. In medical and immunological research, animal models of intestinal diseases are established key tools to investigate the mucosal immune system, colitis and cancer development in the gut. Moreover, such models represent valid systems for testing of novel drugs. In the past, mice had to be killed in order to analyze colitis activity and tumor development. The following protocol describes a method to perform high resolution endoscopic monitoring of live mice. Mice developing colitis or colonic tumors are anesthetized and examined with a miniendoscope. The endoscope is introduced via the anus and the colon is carefully insufflated with an air pump. Endoscopic pictures obtained are of high quality and allow the monitoring and grading of tumors and inflammation. In addition, colonic biopsies can be taken. This protocol can be completed within 1 h.