Identification of two mutually exclusive groups after long-term monitoring of HIV DNA 2-LTR circle copy number in patients on HAART
Identification of two mutually exclusive groups after long-term monitoring of HIV DNA 2-LTR circle copy number in patients on HAART
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DOI：
10.1097/00002030-200303280-00005
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发表时间：
2003-03-28
期刊：
影响因子：
3.8
通讯作者：
Shaunak, S
中科院分区：
文献类型：
--
作者：
Morlese, J;Teo, IA;Shaunak, S
背景：抗逆转录病毒药物疗法可减少但不能消除艾滋病毒在全身的传播和复制。在一些定点研究中，已表明在血浆艾滋病毒核糖核酸（HIV RNA）检测不到的部分患者体内，艾滋病毒DNA的2 - 长末端重复序列（2 - LTR）环持续存在。然而，该环拷贝数随时间的波动程度尚未确定。
方法：建立了一种可靠、可重复且稳健的基于LightCycler定量聚合酶链反应（LC qPCR）的外周血单个核（PBMN）细胞中艾滋病毒DNA的2 - LTR环检测方法。对接受抗逆转录病毒治疗且血浆艾滋病毒RNA检测不到的艾滋病毒1型（HIV - 1）阳性患者进行了这些环的前瞻性纵向研究。
Background: Anti-retroviral drug therapy reduces but does not abolish HIV transmission and replication throughout the body. HIV DNA 2-long terminal repeat (2-LTR) circles have been shown in point-based studies to persist in some patients whose plasma HIV RNA was undetectable. However, the degree of fluctuation of circle copy number over time has not been determined.Methods: A reliable, reproducible and robust quantitative LightCycler (LC qPCR)-based assay for HIV DNA 2-LTR circles in peripheral blood mononuclear (PBMN) cells was established. A prospective, longitudinal study of these circles was undertaken in HIV-1-positive patients on anti-retroviral therapy whose plasma HIV RNA was undetectable at