Host range and cytopathogenicity of the highly attenuated MVA strain of vaccinia virus: Propagation and generation of recombinant viruses in a nonhuman mammalian cell line
Host range and cytopathogenicity of the highly attenuated MVA strain of vaccinia virus: Propagation and generation of recombinant viruses in a nonhuman mammalian cell line
复制标题
DOI：
10.1006/viro.1997.8845
复制
发表时间：
1997-11-24
期刊：
影响因子：
3.7
通讯作者：
Moss, B
中科院分区：
文献类型：
--
作者：
Carroll, MW;Moss, B
改性安卡拉牛痘病毒（MVA）在原代鸡胚成纤维细胞（CEF）中经500多代减毒，目前被用作一种安全的表达载体。我们比较了MVA和亲本安卡拉菌株在CEF和15个永久细胞系中的宿主范围。细胞可分为三类：受纳细胞、半受纳细胞和非受纳细胞。对于MVA，允许类别包括原代CEF，衍生自QT6的鹌鹑细胞系和叙利亚仓鼠细胞系BHK-21。只有在BHK-21细胞中，病毒产量接近原代CEF。半许可类别包括两种非洲绿猴细胞系：BS-C-1和CV-1。MVA的非许可类别包括三种人类细胞系HeLa、293和SW 839；恒河猴细胞系FRhK-4；两种中国仓鼠细胞系CHO和CHL；一个猪细胞系PK(15)；以及三种兔细胞系RK13、rabb - b和SIRC。具有恢复K1L宿主范围基因的MVA的分组相似，除了在允许系中包含RK13细胞外。然而，安卡拉菌株的分组与更宽容和半宽容的细胞系有很大不同。然而，在允许MVA的细胞中，病毒复制到比安卡拉更高的水平，这与与MVA适应相关的正负生长因素相一致。这些细胞系还根据它们对MVA诱导的细胞病变的易感性、重组MVA调控的迟启动子报告基因的表达以及病毒粒子形态发生被阻断的阶段进行了表征。最后，允许的BHK-21细胞系被证明能够构建和繁殖重组MVA，为原代CEF提供了一种替代方案。(C) 1997学术出版社。
Modified vaccinia virus Ankara (MVA), attenuated by over 500 passages in primary chick embyro fibroblasts (CEF), is presently being used as a safe expression vector. We compared the host ranges of MVA and the parental Ankara strain in CEF and 15 permanent cell lines. The cells could be grouped into three categories: permissive, semipermissive, and nonpermissive. For MVA, the permissive category consisted of primary CEF, a quail cell line derived from QT6, and the Syrian hamster cell line BHK-21. Only in BHK-21 cells did the Virus yield approach that occurring in primary CEF. The semipermissive category included two African green monkey cell lines: BS-C-1 and CV-1. The nonpermissive category for MVA consisted of three human cell lines HeLa, 293, and SW 839; one rhesus monkey cell line FRhK-4; two Chinese hamster cell lines CHO and CHL; one pig cell line PK(15); and three rabbit cell lines RK13, RAB-B, and SIRC. The grouping for MVA with a restored K1L host range gene was similar except for the inclusion of RK13 cells among permissive lines. The grouping for the Ankara strain, however, was quite different with more permissive and semipermissive cell lines. Nevertheless, in cells that were permissive for MVA, the virus replicated to higher levels than Ankara, consistent with both positive and negative growth elements associated with the adaptation of MVA. The cell lines were also characterized according to their susceptibilty to MVA-induced cytopathic effects, expression of a late promoter regulated reporter gene by an MVA recombinant, and stage at which virion morphogenesis was blocked, Finally, the permissive BHK-21 cell line was shown to be competent for constructing and propagating recombinant MVA, providing an alternative to primary CEF. (C) 1997 Academic Press.