Members of the neuropeptide transcriptional network in Helicoverpa armigera and their expression in response to light stress
Members of the neuropeptide transcriptional network in Helicoverpa armigera and their expression in response to light stress
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棉铃虫神经肽转录网络的成员及其响应光胁迫的表达
DOI：
10.1016/j.gene.2018.05.070
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Zhu Fen
中科院分区：
文献类型：
--
作者：
Wang Lijun;Liu Xinhui;Liu Zhengxing;Wang Xiaoping;Lei Chaoliang;Zhu Fen
神经肽和肽激素在调节各类昆虫的生理和行为中起着核心作用。夜间的人造光是环境压力的一种形式，最近被认为是夜行昆虫的光压力来源。由于缺乏相关的基因组信息，夜行昆虫神经肽对光胁迫反应的分子生物学研究受到限制。基于对棉铃虫头部转录组的从头测序，我们获得了124,960个单基因。其中，注释为神经肽和肽激素、神经递质前体加工酶和神经递质受体的单基因数量分别为34个、17个和58个。在光胁迫下，qRT-PCR检测的基因表达存在性别差异。在光胁迫下，IMFamide、leuckinin和sNPF基因在雄性的mRNA水平上有差异表达，而在雌性中无差异表达。该结果为棉铃虫神经肽转录网络的多样性提供了新的认识。此外，一些神经肽在光胁迫下表现出性别特异性的差异表达。总的来说，这些结果不仅扩大了已知昆虫神经肽的目录，而且为它们在夜间蛾的光应激反应行为中所起的生理作用的未来功能研究提供了框架。
Neuropeptides and peptide hormones play central roles in the regulation of various types of insect physiology and behavior. Artificial light at night, a form of environmental stress, has recently been regarded as a source of light stress on nocturnal insects. Because related genomic information is not available, molecular biological studies on the response of neuropeptides in nocturnal insects to light stress are limited. Based on the de novo sequencing of the Helicoverpa armigera head transcriptome, we obtained 124,960 unigenes. Of these, the number of unigenes annotated as neuropeptides and peptide hormones, neurotransmitter precursor processing enzymes, and neurotransmitter receptors were 34, 17, and 58, respectively. Under light stress, there were sex-specific differences in gene expression measured by qRT-PCR. The IMFamide, leucokinin and sNPF genes were differentially expressed at the mRNA level in males but not in females in response to light stress. The results provide new insights on the diversity of the neuropeptide transcriptional network ofH.armigera. In addition, some neuropeptides exhibited sex-specific differential expression in response to light stress. Taken collectively, these results not only expand the catalog of known insect neuropeptides but also provide a framework for future functional studies on the physiological roles they play in the light stress response behavior of nocturnal moths.