Radiolabeled BMIPP for imaging hepatic fatty acid metabolism: evaluation of hepatic distribution and metabolism in mice at various metabolic statuses induced by fasting in comparison with palmitic acid.
Radiolabeled BMIPP for imaging hepatic fatty acid metabolism: evaluation of hepatic distribution and metabolism in mice at various metabolic statuses induced by fasting in comparison with palmitic acid.
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用于肝脏脂肪酸代谢成像的放射性标记 BMIPP：与棕榈酸相比，评估禁食诱导的各种代谢状态下小鼠的肝脏分布和代谢。
DOI：
10.2310/7290.2014.00058
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发表时间：
2015
影响因子：
2.8
通讯作者：
Y. Kuge
中科院分区：
文献类型：
--
作者：
Kazuaki Yamasaki;Songji Zhao;Mie Nishimura;Yan Zhao;Wenwen Yu;Yoichi Shimizu;K. Nishijima;N. Tamaki;Hiroshi Takeda;Y. Kuge
肝脏脂肪酸代谢异常与多种疾病有关。为了阐明123 I-15-（p-iodophenyl）-3（R，S）-methylpentadecanoic acid（[123 I]BMIPP）在肝脏脂肪酸代谢显像中的应用，我们测定了禁食诱导的小鼠在不同代谢状态下[125 I]BMIPP的肝脏分布/代谢，并与[1-（14）C]棕榈酸（[1-（14）C]PA）的结果进行了比较。给进食或禁食（禁食6、12和24小时）小鼠静脉注射[125 I]BMIPP或[1-（14）C]PA。在注射后1 - 120分钟测量肝脏放射性（n = 5 - 15/时间点），并通过薄层色谱法分析放射性脂质代谢产物（n = 3/时间点）。 计算曲线下面积（AUC）。在给予[125 I]BMIPP的小鼠中，肝脏放射性随禁食时间增加（AUC：35.1、45.5、57.6和59.0 [%注射剂量（ID）/g/kg]，进食、6、12和24小时禁食）。在给予[1-（14）C]PA（100.6、101.0、116.5和121.5 [%ID/g/kg] μ min）的小鼠中观察到相似的特征性变化。代谢物分析显示，两组中的二甲双胍分数均随禁食而增加（[125 I]BMIPP组为5.7、12.8、32.0和37.9 [%ID/g/kg] μ min;[1-（14）C]PA组为20.6、39.2、66.0和67.9 [%ID/g/kg] μ min）。因此，[125 I]BMIPP证实了禁食诱导的肝脏脂肪酸代谢的变化，表明[123 I]BMIPP用于肝脏脂肪酸代谢成像的潜力。
Abnormalities in hepatic fatty acid metabolism are involved in various diseases. In order to clarify the use of 123I-15-(p-iodophenyl)-3(R,S)-methylpentadecanoic acid ([123I]BMIPP) for imaging hepatic fatty acid metabolism, we determined the hepatic distribution/metabolism of [125I]BMIPP in mice at various metabolic statuses induced by fasting, and compared the results with those of [1-(14)C]palmitic acid ([1-(14)C]PA). Fed or fasted (6, 12, and 24 hour-fasted) mice were intravenously injected with [125I]BMIPP or [1-(14)C]PA. Hepatic radioactivity was measured at 1 to 120 minutes after the injection (n = 5 to 15/time points), and radioactive lipid metabolites were analyzed by thin-layer chromatography (n = 3/time points). The areas under the curves (AUCs) were calculated. In mice given [125I]BMIPP, the hepatic radioactivity was increased with the fasting time (AUC: 35.1, 45.5, 57.6, and 59.0 [% injected dose (ID)/g/kg]▪min for fed, 6, 12, and 24 hour-fasted). Similar characteristic changes were observed in mice given [1-(14)C]PA (100.6, 101.0, 116.5, and 121.5 [%ID/g/kg]▪min). Metabolite analysis showed that the triglyceride-fraction was increased by fasting in both groups (5.7, 12.8, 32.0, and 37.9 [%ID/g/kg]▪min for [125I]BMIPP groups; 20.6, 39.2, 66.0, and 67.9 [%ID/g/kg]▪min for [1-(14)C]PA groups). Thus, [125I]BMIPP demonstrated the changes in hepatic fatty acid metabolism induced by fasting, indicating the potential of [123I]BMIPP for imaging hepatic fatty acid metabolism.
影响因子：
15.9
作者：
Donnelly, KL;Smith, CI;Parks, EJ
通讯作者：
Parks, EJ
DOI：
10.1172/jci22422
发表时间：
2004-07
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
J. Browning;J. Horton
通讯作者：
J. Browning;J. Horton