Color discriminating retinal configurations of sensory rhodopsin I by photo-irradiation solid state NMR spectroscopy.
Color discriminating retinal configurations of sensory rhodopsin I by photo-irradiation solid state NMR spectroscopy.
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通过光照射固态核磁共振光谱法对感觉视紫红质 I 进行颜色辨别的视网膜构型。
DOI：
10.1002/ange.201309258
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
A.
中科院分区：
文献类型：
--
作者：
Yornoda;H.;Makino;Y.;Tomonaga;Y.;Hidaka;T.;Kawamura;I.;Okitsu;T.;Wada;A.;Sudo;Y.;Naito;A.
感觉视紫红质I（sensory rhodopsin I，SRI）可区分多种颜色，用于引诱和排斥趋光性。 旨在揭示颜色依赖机制的研究表明，SRI不仅在光生物学中而且在光化学中都是一个具有挑战性的系统。在SRI的光反应过程中，M-中间体（引诱剂）通过吸收蓝光转化为P-中间体（驱避剂）。因此，SRI然后循环回到G状态。使用原位光辐照固态NMR光谱，用[20 - 13 C]retnal-SrSRI的13 C NMR信号监测光反应。 在-40 °C下通过520 nm的照射捕获M-中间体。 通过随后在365 nm下照射将其转化为P-中间体。 这些结果表明，通过365 nm的照射，G-态可以直接转化为P-中间体。 因此，固定捕获的M-和P-中间体分别负责正和负趋光性。
SRI (sensory rhodopsin I) can discriminate multiple colors for the attractant and repellent phototaxis. Studies aimed at revealing the color‐dependent mechanism show that SRI is a challenging system not only in photobiology but also in photochemistry. During the photoreaction of SRI, an M‐intermediate (attractant) transforms into a P‐intermediate (repellent) by absorbing blue light. Consequently, SRI then cycles back to the G‐state. The photoreactions were monitored with the13C NMR signals of [20‐13C]retnal‐SrSRI using in situ photo‐irradiation solid‐state NMR spectroscopy. The M‐intermediate was trapped at −40 °C by illumination at 520 nm. It was transformed into the P‐intermediate by subsequent illumination at 365 nm. These results reveal that the G‐state could be directly transformed to the P‐intermediate by illumination at 365 nm. Thus, the stationary trapped M‐ and P‐intermediates are responsible for positive and negative phototaxis, respectively.