Free, glucuronide, and sulfate catecholamines in the rat: effect of hypoxia.
Free, glucuronide, and sulfate catecholamines in the rat: effect of hypoxia.
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大鼠体内的游离儿茶酚胺、葡萄糖醛酸苷和硫酸儿茶酚胺：缺氧的影响。
DOI：
10.1152/jappl.1985.59.1.12
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发表时间：
1985
影响因子：
3.3
通讯作者：
L. Peyrin
中科院分区：
文献类型：
--
作者：
J. Claustre;R. Favre;J. Cottet;L. Peyrin
本文研究了清醒大鼠缺氧（5.5- 6%O_2，4 h）交感神经刺激后结合型儿茶酚胺（CA）的形成和排泄。缺氧引起游离肾上腺素（E，X12）和去甲肾上腺素（NE，X6）迅速而强烈的增加，而游离多巴胺（DA，X2）的增加有限。E和NE的硫酸盐结合物具有与游离形式相似的动力学，而葡萄糖醛酸苷仅中度和最近改变。与游离和硫酸化DA相比，主要血浆结合物DA葡糖苷酸减少（-25%）。这一结果表明，DA葡萄糖醛酸苷，不像其他CA共轭物，是不相关的解毒，但可能提供CA前体。尿液结合物严重反映血浆结合物。在含氧量正常的对照组中，CA结合物在血浆中占主导地位，而游离胺在尿液中占主导地位。缺氧主要增加E和NE葡萄糖醛酸苷的排泄，但不增加游离胺的排泄。尿DA（游离或结合）降低（-25%），结果仅与血浆DA葡萄糖醛酸苷一致。血浆和尿中的儿茶酚胺之间的关系较差，这表明肾脏在CA处理中的重要性。
The formation and excretion of conjugated catecholamines (CA) was studied in conscious rats after sympathetic stimulation by hypoxia (5.5-6% O2, 4 h). Hypoxia induced a rapid and intense increase of free epinephrine (E, X 12) and norepinephrine (NE, X 6) but only a limited enhancement of free dopamine (DA, X 2). Sulfate conjugates of E and NE had kinetics similar to the free forms, while glucuronides were only moderately and lately altered. In contrast to free and sulfated DA, DA glucuronide, the major plasma conjugate, was decreased (-25%). This result suggests that DA glucuronide, unlike other CA conjugates, is not related to detoxication but might supply a CA precursor. Urinary conjugates badly reflected plasma conjugates. In normoxic controls, CA conjugates prevailed in the plasma, whereas the free amines prevailed in the urine. Hypoxia increased mainly the excretion of E and NE glucuronide but not of the free amines. Urinary DA, free or conjugated, was decreased (-25%), a result in keeping with plasma DA glucuronide only. The poor relations between plasma and urine catecholamines pinpoint the importance of the kidney in CA handling.