PAPILLOMAVIRUS DNA IN HUMAN TONGUE CARCINOMAS
PAPILLOMAVIRUS DNA IN HUMAN TONGUE CARCINOMAS
复制标题
DOI：
10.1002/ijc.2910360510
复制
发表时间：
1985-01-01
影响因子：
6.4
通讯作者：
ZURHAUSEN, H
中科院分区：
文献类型：
--
作者：
DEVILLIERS, EM;WEIDAUER, H;ZURHAUSEN, H
七个活检从不同的舌癌进行了分析，人乳头状瘤病毒（HPV）序列的Southern印迹分析。与各种类型的人乳头瘤病毒杂交后，发现1个肿瘤呈阳性。在高度严格的条件下，来自一个肿瘤的DNA与HPY 2 DNA杂交，而另外2个阳性活检标本含有HPV 16 DNA。所有阳性肿瘤均显示相应DNA的高拷贝数。HPV-2阳性肿瘤的裂解模式与已建立的HPV 2原型不同。在一个HPV-16阳性舌癌中也观察到与HPV-16原型的微小差异。
Seven biopsies from different carcinomas of the tongue were analyzed for human papilloma virus (HPV) sequences by Southern blot analysis. After hybridization with various types of human papilloma viruses, 1 tumors were found to be positive. Whereas DNA from one tumor hybridized with HPY 2 DNA under conditions of high stringency, the 2 other positive biopsies contained HPV 16 DNA. All positive tumors revealed high copy numbers of the respective DNA. The cleavage pattern of the HPV‐2‐positive tumor differed from the established HPV 2 prototypes. Minor differences from the HPV 16 prototype were also noted in one of the HPV‐16‐positive tongue carcinomas.