The Stage of the Estrus Cycle Is Critical for Interpretation of Female Mouse Social Interaction Behavior
The Stage of the Estrus Cycle Is Critical for Interpretation of Female Mouse Social Interaction Behavior
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DOI：
10.3389/fnbeh.2020.00113
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发表时间：
2020-06-30
影响因子：
3
通讯作者：
Fagiolini, Michela
中科院分区：
文献类型：
--
作者：
Chari, Trishala;Griswold, Sophie;Fagiolini, Michela
传统上，生物医学研究中的雌性动物被排除在研究之外，因为发情周期会增加研究的复杂性。然而，鉴于性别差异在各种神经系统疾病中的重要性，测试雌性小鼠对于确定疾病中的性连锁效应至关重要。为了确定简单行为对发情周期中激素波动的敏感性，我们研究了性别和发情周期对小鼠表型实验室中常用的各种行为任务的影响。雄性和雌性C57 BL/6 J小鼠在一小组短期试验中进行试验，每次试验完成后，立即使用阴道灌洗液的细胞学将雌性小鼠分类为性接受（发情前期和发情期）或非接受（NR;发情后期和间情期）。我们发现，有一个显着的差异，在3室的社会互动（SI）之间的雌性小鼠在其发情周期的不同阶段，与性接受小鼠没有表现出优先兴趣，在一个新的雌性小鼠相比，一个空的房间。NR雌性小鼠对新型雌性小鼠的偏好程度与雄性小鼠对新型雄性小鼠的偏好程度相同。在焦虑高架迷宫（迷宫;孔板）、工作记忆[新物体识别（NOR）]和运动学习（旋转杆重复测试）测试中，性别之间或性别内均无差异。我们的结论是，发情周期的阶段可能会影响SI之间的同性同种，并不影响性能的高架十字迷宫，孔板，NOR，和旋转棒。
Female animals in biomedical research have traditionally been excluded from research studies due to the perceived added complexity caused by the estrus cycle. However, given the importance of sex differences in a variety of neurological disorders, testing female mice is critical to identifying sex-linked effects in diseases. To determine the susceptibility of simple behaviors to hormonal fluctuations in the estrus cycle, we studied the effects of sex and the estrus cycle on a variety of behavioral tasks commonly used in mouse phenotyping laboratories. Male and female C57BL/6J mice were tested in a small battery of short duration tests and, immediately on completion of each test, females were classified using cytology of vaginal lavages as sexually-receptive (proestrus and estrus) or non-receptive (NR; metestrus and diestrus). We showed that there was a significant difference in 3-chamber social interaction (SI) between female mice at different stages of their estrus cycle, with sexually-receptive mice showing no preferential interest in a novel female mouse compared with an empty chamber. NR female mice showed the same level of preference for a novel female mouse as male mice did for a novel male mouse. No differences between or within sexes were found for tests of anxiety elevated plus maze (EPM; Hole board), working memory [Novel object recognition (NOR)], and motor learning (repeated tests on rotarod). We conclude that the stage of the estrus cycle may impact SI between same-sex conspecifics, and does not impact performance in the elevated plus-maze, hole board, NOR, and rotarod.