Reduction of the fraction of circulating helper-inducer T cells identified by monoclonal antibodies in psoriatic patients treated with long-term psoralen/ultraviolet-A radiation (PUVA).
Reduction of the fraction of circulating helper-inducer T cells identified by monoclonal antibodies in psoriatic patients treated with long-term psoralen/ultraviolet-A radiation (PUVA).
复制标题
在接受长期补骨脂素/紫外线 A 辐射 (PUVA) 治疗的银屑病患者中，单克隆抗体识别的循环辅助诱导 T 细胞比例减少。
DOI：
10.1111/1523-1747.ep12500058
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Colvin,RB
中科院分区：
文献类型：
--
作者：
Moscicki,RA;Morison,WL;Parrish,JA;Bloch,KJ;Colvin,RB
已发现紫外线辐射会改变人类淋巴细胞的分布和功能。为了确定光化疗 (PUVA) 是否改变携带 T 细胞亚群标记物的淋巴细胞的循环水平，对来自接受 PUVA 治疗数年（平均 4.6 ± 1.4 年）的 9 名银屑病患者、17 名未经治疗的活动性银屑病患者和 20 名健康志愿者的细胞进行了 T 细胞表面标记物单克隆抗体的反应，包括 OKT3（所有外周血） T 细胞）、OKT4（辅助/诱导 T 细胞）、OKT6（普通胸腺细胞）和 OKT8 抑制/细胞毒性 T 细胞），并通过流式细胞术进行分析。健康志愿者和活动性银屑病患者之间的 T 细胞亚群分布没有差异。相比之下，与健康志愿者或未接受 PUVA 治疗的活动性银屑病患者相比，接受 PUVA 治疗的患者与 OKT3 和 OKT4 反应的淋巴细胞百分比较低（分别降低 16% 和 12%，p < 0.0025。OKT8 和 OKT6 携带细胞的百分比没有差异。随后，三分之二的人发展为皮肤鳞状细胞癌 接受 PUVA 治疗的患者 T4 携带细胞百分比最低。这些发现表明，长期 PUVA 治疗与循环辅助/诱导 T 细胞的减少有关。这种减少可能与 PUVA 治疗患者免疫功能的改变有关。
Ultraviolet radiation has been found to alter the distribution and function of human lymphocytes. To determine whether photochemotherapy (PUVA) alters circulating levels of T cell subset marker-bearing lymphocytes, cells from 9 patients with psoriasis undergoing PUVA therapy for several years (mean 4.6 ± 1.4 yr), 17 patients with active untreated psoriasis, and 20 healthy volunteers were reacted with monoclonal antibodies to T cell surface markers, including OKT3 (all peripheral blood T cells), OKT4 (helper/inducer T cells), OKT6 (common thymocytes), and OKT8 suppressor/cytotoxic T cells), and analyzed by flow cytometry. There were no differences in the distribution of T cell subsets between healthy volunteers and patients with active psoriasis. In contrast, the percentages of lymphocytes reacting with OKT3 and OKT4 were lower (by 16% and 12% percent respectively,p< 0.0025 in the PUVA-treated patients compared to healthy volunteers or patients with active psoriasis that had not received PUVA therapy. There was no difference in the percentage of OKT8 and OKT6 bearing cells. Squamous cell carcinoma of the skin subsequently developed in 2 of 3 PUVA-treated patients with the lowest percentages of T4-bearing cells. These findings indicate that long-term PUVA therapy is associated with a reduction in circulating helper/inducer T cells. This reduction may have a role in the altered immune function reported in PUVA-treated patients.