Integrated optical coherence tomography and optical coherence microscopy imaging of ex vivo human renal tissues.
Integrated optical coherence tomography and optical coherence microscopy imaging of ex vivo human renal tissues.
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DOI：
10.1016/j.juro.2011.09.149
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发表时间：
2012-02
期刊：
影响因子：
--
通讯作者：
Connolly JL
中科院分区：
文献类型：
--
作者：
Lee HC;Zhou C;Cohen DW;Mondelblatt AE;Wang Y;Aguirre AD;Shen D;Sheikine Y;Fujimoto JG;Connolly JL
我们评估了使用光学相干断层扫描和光学相干显微镜技术来评估人类肾脏形态的可行性。手术切除后，对19例患者的35个肾脏标本进行了体外成像，其中包括12个正常组织和23个肿瘤（16个透明细胞肾细胞癌，5个乳头状肾细胞癌和2个嗜酸细胞瘤）。将光学相干断层扫描和光学相干显微镜图像与相应的苏木精和伊红组织学进行比较，以识别正常和病理肾组织的特征。三名对组织学不知情的病理学家评估了光学相干显微镜图像区分正常肾组织和肿瘤肾组织的灵敏度和特异性。光学相干断层扫描和光学相干显微镜图像显示正常肾脏的建筑特征，包括肾小球，曲小管，集合管和亨利袢。每种肾肿瘤成像方法都清楚地显示了形态学变化和成像深度降低。光学相干断层扫描和显微镜特征与相应的组织学匹配良好。当使用光学相干显微镜图像评价正常与肿瘤标本时，三名观察者分别达到88%、100%和100%的灵敏度，以及100%、88%和100%的特异性，观察者间基本一致（κ = 0.82，p <0.01）。集成的光学相干断层扫描和光学相干显微镜成像可提供真实的实时肾脏病理学的共配准、多尺度图像，无需外源性造影剂或组织学处理。高灵敏度和特异性，实现了使用光学相干显微镜区分正常与肿瘤性肾组织，这表明可能的应用指导肾肿块活检或评估手术切缘。
We evaluated the feasibility of using optical coherence tomography and optical coherence microscopy technology to assess human kidney morphology. A total of 35 renal specimens from 19 patients, consisting of 12 normal tissues and 23 tumors (16 clear cell renal cell carcinomas, 5 papillary renal cell carcinomas and 2 oncocytomas) were imaged ex vivo after surgical resection. Optical coherence tomography and optical coherence microscopy images were compared to corresponding hematoxylin and eosin histology to identify characteristic features of normal and pathological renal tissues. Three pathologists blinded to histology evaluated the sensitivity and specificity of optical coherence microscopy images to differentiate normal from neoplastic renal tissues. Optical coherence tomography and optical coherence microscopy images of normal kidney revealed architectural features, including glomeruli, convoluted tubules, collecting tubules and loops of Henle. Each method of imaging renal tumors clearly demonstrated morphological changes and decreased imaging depth. Optical coherence tomography and microscopy features matched well with the corresponding histology. Three observers achieved 88%, 100% and 100% sensitivity, and 100%, 88% and 100% specificity, respectively, when evaluating normal vs neoplastic specimens using optical coherence microscopy images with substantial interobserver agreement (κ = 0.82, p <0.01). Integrated optical coherence tomography and optical coherence microscopy imaging provides coregistered, multiscale images of renal pathology in real time without exogenous contrast medium or histological processing. High sensitivity and specificity were achieved using optical coherence microscopy to differentiate normal from neoplastic renal tissues, suggesting possible applications for guiding renal mass biopsy or evaluating surgical margins.
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