Maturation of osteoblast-like SaoS2 induced by carbon nanotubes
Maturation of osteoblast-like SaoS2 induced by carbon nanotubes
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DOI：
10.1088/1748-6041/4/1/015005
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发表时间：
2009-02-01
影响因子：
4
通讯作者：
Watari, Fumio
中科院分区：
文献类型：
--
作者：
Li, Xiaoming;Gao, Hong;Watari, Fumio
成骨细胞的成骨成熟对于骨形成至关重要。在这项研究中，多壁碳纳米管（MWCNT）和石墨（GP）被压制成压坯。与石墨相比，碳纳米管吸附蛋白质的能力更强。培养人成骨细胞样 SaoS2 细胞，并在体外比较细胞对两种致密物的反应。同时，我们使用培养板上的细胞培养物作为对照。对样品中培养的细胞的骨连接蛋白、骨桥蛋白和骨钙蛋白基因表达、总蛋白（TP）量、碱性磷酸酶活性（ALP）和DNA进行了测定。在常规培养过程中，发现碳纳米管上的骨连接素、骨桥蛋白和骨钙素基因表达水平、ALP/DNA和TP/DNA显着升高。为了证实吸附在碳纳米管上的大量特定蛋白质对此至关重要的假设，在细胞培养之前将压块预先浸泡在含有额外重组人骨形态发生蛋白-2 (rhBMP-2) 的培养基中。与GP相比，将压块预浸泡在含有rhBMP-2的培养基中后，MWCNT上测试细胞的骨连接素、骨桥蛋白和骨钙素基因表达水平、ALP/DNA和TP/DNA增加更多。结果表明，碳纳米管可能通过吸附更特异性的蛋白质来诱导成骨细胞的成骨成熟。
Osteogenic maturation of the osteoblast is crucial for bone formation. In this study, multi-walled carbon nanotubes (MWCNTs) and graphite (GP) were pressed as compacts. The greater ability of carbon nanotubes to adsorb proteins, compared with graphite, was shown. Human osteoblast-like SaoS2 cells were cultured and the cell response to the two kinds of compacts was compared in vitro. Meanwhile, we used cell culture on the culture plate as a control. Assays for osteonectin, osteopontin and osteocalcin gene expression, total protein (TP) amount, alkaline phosphatase activity (ALP) and DNA of cells cultured on the samples were done. During the conventional culture, significantly higher osteonectin, osteopontin and osteocalcin gene expression level, ALP/DNA and TP/DNA on carbon nanotubes were found. To confirm the hypothesis that the larger amount of specific proteins adsorbed on the carbon nanotubes was crucial for this, the compacts were pre-soaked in culture medium having additional recombinant human bone morphogenetic protein-2 (rhBMP-2) before cell culture. Compared with GP, osteonectin, osteopontin and osteocalcin gene expression level, ALP/DNA and TP/DNA of the cells tested increased more on the MWCNTs after the compacts were pre-soaked in the culture medium with rhBMP-2. The results indicated that the carbon nanotubes might induce osteogenic maturation of the osteoblast by adsorbing more specific proteins.