CELL LOCOMOTION FORCES VERSUS CELL CONTRACTION FORCES FOR COLLAGEN LATTICE CONTRACTION - AN INVITRO MODEL OF WOUND CONTRACTION
CELL LOCOMOTION FORCES VERSUS CELL CONTRACTION FORCES FOR COLLAGEN LATTICE CONTRACTION - AN INVITRO MODEL OF WOUND CONTRACTION
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DOI：
10.1016/0040-8166(90)90070-p
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发表时间：
1990-01-01
期刊：
影响因子：
2.6
通讯作者：
RAJARATNAM, JBM
中科院分区：
文献类型：
--
作者：
EHRLICH, HP;RAJARATNAM, JBM
培养的人皮肤成纤维细胞悬浮在快速聚合的胶原蛋白基质中产生成纤维细胞填充的胶原蛋白晶格。随着时间的推移，这种晶格将经历称为晶格收缩的尺寸减小。在这个过程中，两个不同的细胞群发展。在网格的外围，高度定向的细胞片，形态学上可识别为肌成纤维细胞，显示细胞间接触和厚的、富含肌动蛋白染色的细胞质应力纤维。有人提出，这些细胞经历细胞收缩产生多细胞收缩单位，重新定向与它们相关的胶原纤维。中心区域的细胞，称为成纤维细胞，是随机取向的，几乎没有细胞与细胞的接触，并微弱染色肌动蛋白胞质丝。相反，有人提出，细胞作为单一单位工作，使用细胞运动力来重新定向与它们相关的胶原纤维。使用这个模型，我们试图确定这两种机制中的哪一种，细胞收缩或细胞运动，负责收缩胶原晶格的力。我们的实验表明，成纤维细胞产生这种收缩力，晶格收缩的机制似乎与细胞运动有关。这与肌成纤维细胞相反;其中收缩机制基于细胞收缩。试图在胶原基质内移动的成纤维细胞重新组织周围的胶原原纤维;当这些胶原原纤维不能进一步组织并且细胞与细胞接触发展时，这首先发生在晶格的外围，这些细胞不再参与晶格收缩的动态方面。
Cultured human dermal fibroblasts suspended in a rapidly polymerizing collagen matrix produce a fibroblast-populated collagen lattice. With time, this lattice will undergo a reduction in size referred to as lattice contraction. During this process, two distinct cell populations develop. At the periphery of the lattice, highly oriented sheets of cells, morphologically identifiable as myofibroblasts, show cell-to-cell contacts and thick, actin-rich staining cytoplasmic stress fibers. It is proposed that these cells undergoing cell contraction produce a multicellular contractile unit which reorients the collagen fibrils associated with them. The cells in the central region, referred to as fibroblasts, are randomly oriented, with few cell-to-cell contacts and faintly staining actin cytoplasmic filaments. In contrast it is proposed that cells working as single units use cell locomotion forces to reorient the collagen fibrils associated with them. Using this model, we sought to determine which of these two mechanisms, cell contraction or cell locomotion, is responsible for the force that contracts collagen lattices. Our experiments showed that fibroblasts produce this contractile force, and that the mechanism for lattice contraction appears to be related to cell locomotion. This is in contrast to a myofibroblast; where the mechanism for contraction is based upon cell contractions. Fibroblasts attempting to move within the collagen matrix reorganize the surrounding collage fibrils; when these collagen fibrils can be organized no further and cell-to-cell contacts develop, which occurs at the periphery of the lattice first, these cells can no longer participate in the dynamic aspects of lattice contraction.