Expression of mast cell tryptase by myeloblasts in a group of patients with acute myeloid leukemia
Expression of mast cell tryptase by myeloblasts in a group of patients with acute myeloid leukemia
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DOI：
10.1182/blood.v98.7.2200
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发表时间：
2001-10-01
期刊：
影响因子：
20.3
通讯作者：
Valent, P
中科院分区：
文献类型：
--
作者：
Sperr, WR;Jordan, JH;Valent, P
α-和β-类胰蛋白酶基因编码由肥大细胞大量表达的丝氨酸蛋白酶。在生理条件下，其他骨髓细胞实际上是类胰蛋白酶阴性的。然而，类胰蛋白酶也在几种髓性白血病细胞系中表达。在这项研究中，通过荧光酶免疫测定法测定了150例急性白血病患者（初治急性髓性白血病[AML]，n = 108;继发性AML，n = 25;急性淋巴细胞白血病[ALL]，n = 17）的血清总类胰蛋白酶水平。在健康受试者（n = 30）中，类胰蛋白酶水平范围为2.0 - 12.6 ng/mL。在108例初治AML患者中的42例（39%）和25例继发性AML患者中的11例（44%）中检测到类胰蛋白酶水平升高（> 15）。ALL患者中未发现类胰蛋白酶水平升高。在初治AML中，在法国-美国-英国分类MO（6/9）、M2（9/14）、M3（4/6）和M4 eo（7/7）的患者中经常检测到类胰蛋白酶水平升高，在M1（7/20）、M4（6/26）、M5（2/18）、M6（0/5）或M7（1/3）中不太常见。在M4 eo中发现最高的类胰蛋白酶水平。骨髓切片抗类胰蛋白酶抗体的免疫组织化学染色以及免疫电镜显示类胰蛋白酶在成髓细胞的细胞质中表达。如通过北方印迹和逆转录酶-聚合酶链反应所评估的，AML细胞表达α-类胰蛋白酶信使RNA（mRNA），但表达很少或不表达β-类胰蛋白酶mRNA。化疗前血清类胰蛋白酶升高的AML患者进入完全缓解后，类胰蛋白酶水平恢复正常或接近正常值。胚细胞持续存在或再生与类胰蛋白酶水平持续升高或复发性升高有关。总之，类胰蛋白酶在一组AML中的成髓细胞中表达，并且可以作为有用的疾病相关标志物。(C)2001年，美国血液学会。
alpha- and beta -tryptase genes encode serine proteases that are abundantly expressed by mast cells. Under physiologic conditions other myeloid cells are virtually tryptase negative. However, tryptases are also expressed In several myeloid leukemia cell lines. In this study, serum total tryptase levels were determined In 150 patients with acute leukemias (de novo acute myeloid leukemia [AML], n = 108; secondary AML, n = 25; acute lymphoid leukemia [ALL], n = 17) by fluoroenzyme immunoassay. In healthy subjects (n = 30), tryptase levels ranged between 2.0 and 12.6 ng/mL. Elevated tryptase levels (> 15) were detected In 42 (39%) of 108 patients with de novo AML and In 11 (44%) of 25 patients with secondary AML. No elevated tryptase levels were found in patients with ALL. In de novo AML, elevated tryptase levels were frequently detected in patients with French-American-British classification MO (6 of 9), M2 (9 of 14), M3 (4 of 6), and M4eo (7 of 7), and less frequently in M1 (7 of 20), M4 (6 of 26), M5 (2 of 18), M6 (0 of 5), or M7 (1 of 3). The highest tryptase levels were found in M4eo. Immunohistochemical staining of bone marrow sections with anti-tryptase antibody as well as immunoelectron microscopy revealed tryptase expression in the cytoplasm of myeloblasts. As assessed by Northern blotting and reverse transcriptase-polymerase chain reaction, AML cells expressed a-tryptase messenger RNA (mRNA) but little or no beta -tryptase mRNA. In AML patients with elevated serum tryptase before chemotherapy, who entered complete remission, tryptase levels returned to normal or near normal values. Blast cell persistence or regrowth was associated with a persistently elevated level or recurrent Increase of tryptase. Together, tryptase Is expressed In myeloblasts in a group of AML and may serve as a useful disease-related marker. (C) 2001 by The American Society of Hematology.