Glycinebetaine increases chilling tolerance and reduces chilling-induced lipid peroxidation in Zea mays L.
Glycinebetaine increases chilling tolerance and reduces chilling-induced lipid peroxidation in Zea mays L.
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DOI：
10.1046/j.1365-3040.2000.00570.x
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发表时间：
2000-06-01
影响因子：
7.3
通讯作者：
Chen, THH
中科院分区：
文献类型：
--
作者：
Chen, WP;Li, PH;Chen, THH
在含有甘氨酸甜菜碱（GB）的培养基中培养的玉米（Zea mays L. cv 'Black Mexican Sweet'）悬浮培养细胞和幼苗的耐冷性提高。氯化三苯四氮唑（TTC）还原试验表明，在4℃下冷却7 d后，在26℃下用1 mm GB处理1 d的细胞存活率（30%）是未处理对照的两倍。在培养基中添加2.5 mM GB可获得最大的耐冷性（40%）。细胞再生试验的结果与TTC法测定的活力一致。在添加不同浓度GB的悬浮培养细胞中，细胞中GB的积累量与培养基中GB的浓度成正比，并在浓度为240 μ mol （g DW）时达到饱和（-1）。低温耐受性的增强程度与细胞内积累的GB含量呈正相关。耐寒性的增加是时间依赖性的；在4℃/2℃的低温下，用2.5 mM GB给幼苗喂食3 d后，它们的抗冷性也得到了提高，这可以证明，在低温下，用GB处理过的细胞与未处理过的细胞相比，丙二醛的产生显著降低了脂质过氧化。这些结果表明，低温耐受性的增加可能部分是由于在GB存在下细胞膜脂质过氧化的减少。
Chilling tolerance was increased in suspension-cultured cells and seedlings of maize (Zea mays L. cv 'Black Mexican Sweet') grown in media containing glycinebetaine (GB). A triphenyl tetrazolium chloride (TTC) reduction test indicated that after a 7 d chilling period at 4 degrees C, cells treated with 1 mm GB at 26 degrees C for 1 d had a survival rate (30%) that was twice as high as that of untreated controls. The addition of 2.5 mM GB to the culture medium resulted in maximum chilling tolerance (40%). The results of a cell regrowth assay were consistent with viability determined by the TTC method. In suspension-cultured cells supplemented with various concentrations of GB, accumulation of GB in the cells was proportional to the GB concentration in the medium and was saturated at a concentration of 240 mu mol (g DW)(-1). The degree of increased chilling tolerance was positively correlated with the level of GB accumulated in the cells. The increased chilling tolerance was time-dependent; i.e. it was first observed 3 h after treatment and reached a plateau after 14 h. Feeding seedlings with 2.5 mM GB through the roots also improved their chilling tolerance, as evidenced by the prevention of chlorosis after chilling for 3 d at 4 degrees C/2 degrees C. Lipid peroxidation, as expressed by the production of malondialdehyde, was significantly reduced in GB-treated cells compared with the untreated controls during chilling. These results suggest that increased chilling tolerance may be due, in part, to the reduction of lipid peroxidation of the cell membranes in the presence of GB.