Full‑length transcriptome analysis reveals the differences between floral buds of recessive genic male‑sterile line (RMS3185A) and fertile line (RMS3185B) of cabbage
Full‑length transcriptome analysis reveals the differences between floral buds of recessive genic male‑sterile line (RMS3185A) and fertile line (RMS3185B) of cabbage
复制标题
全长转录组分析揭示了甘蓝隐性雄性不育系（RMS3185A）和可育系（RMS3185B）花芽之间的差异
DOI：
10.1007/s00425-020-03542-8
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发表时间：
2021
期刊：
影响因子：
4.3
通讯作者：
Zhuoyue Cui
中科院分区：
文献类型：
--
作者：
Aimei Tian;Enhui Zhang;Zhuoyue Cui
主要结论淀粉和蔗糖代谢以及植物与病原菌互作途径在甘蓝隐性核不育中起主导作用。RGMS在植物中很常见，已被广泛应用于许多作物的杂交种子生产中，作为一种有效和经济的系统。然而，关于甘蓝RGMS的分子机制知之甚少。因此，在自发RGMS突变体RMS 3185 A及其近等基因可育系（NIL）RMS 3185 B的小（直径< 1.6 mm）、中等（直径约2.5 mm）和大花芽（直径约3.4 mm）中进行全长转录组学和生理学分析，以鉴定与RMGS相关的差异表达基因（DEG）。RMS 3185 B和RMS 3185 A的花粉异常出现在大花蕾期。与RMS 3185 B相比，RMS 3185 A的成熟花药和雄蕊短于RMS 3185 B，花药不开裂。葡萄糖、果糖、海藻糖、淀粉和纤维素的含量在RMS 3185 A中均显著低于RMS 3185 B的大花蕾。PacBio测序结果表明，DEGs主要集中在大花芽期。结合KEGG富集分析，GO中与细胞壁、花粉和花药、戊糖和葡萄糖醛酸相互转化（ko 00040）、淀粉和蔗糖代谢（ko 00500）以及植物-病原菌相互作用（ko 04626）相关的DEG显著富集。其中，ko 00040中涉及18个PEI、22个PEL、3个PG和15个PGL的细胞壁/果胶相关基因，ko 00500中涉及1个UGDH、1个SPS、4个CWINV、4个TPP/TPS和4个EGL的细胞壁/果胶相关基因，以及16个钙依赖蛋白激酶（CDPK）、1个环核苷酸门控离子通道（CNGC）和23个钙结合蛋白CML（CML）的植物-病原互作基因，在RMS 3185 A中相对于在RMS 3185 B中显著下调。此外，与ko 04626相关的两个CM和一个转录因子WRKY 33在RMS 3185 A中的表达较RMS 3185 B中的表达上调。综上所述，我们推测这些基因的表达变化与钙信号和糖代谢有关，从而影响甘蓝RGMS的发生。
Main conclusionStarch and sucrose metabolism and plant–pathogen interaction pathways play a dominate role in recessive genic male sterility (RGMS) of cabbage (Brassica oleraceaL. var.capitata).AbstractRGMS is common in plants and has been widely applied as an effective and economic system for hybrid seed production in many crops. However, little is known regarding the molecular mechanisms of RGMS in cabbage. Hence, full-length transcriptomic and physiological analysis were performed in the spontaneous RGMS mutant RMS3185A and its near-isogenic fertile line (NIL) RMS3185B of small (< 1.6 mm in diameter), medium (~ 2.5 mm in diameter), and large floral buds (~ 3.4 mm in diameter) to identify the differentially expressed genes (DEGs) associated with RMGS. The pollen abnormalities between RMS3185B and RMS3185A appeared at the large floral bud stage. In contrast with RMS3185B, the mature anthers and stamens of RMS3185A were shorter than those of RMS3185B, and the anthers did not dehiscent. The concentrations of glucose, fructose, trehalose, starch, and cellulose in RMS3185A were all significantly lower than those in large floral buds of RMS3185B. PacBio sequencing results showed that DEGs were mainly concentrated in large floral bud stage. In combination with the KEGG enrichment analysis of DEGs in GO terms related to cell wall, pollen and anther, pentose and glucuronate interconversions (ko00040), starch and sucrose metabolism (ko00500), and plant–pathogen interaction (ko04626) were significantly enriched. Among which, cell-wall/pectin-related genes of eighteenPEI, twenty-twoPEL, threePG, and fifteenPGLinvolved in ko00040, and oneUGDH, oneSPS, fourCWINV, fourTPP/TPS, and fourEGLinvolved in ko00500, as well as plant-pathogen interaction genes, including sixteen calcium-dependent protein kinase (CDPK), one cyclic nucleotide-gated ion channel (CNGC), and twenty-three calcium-binding protein CML (CML), were significantly down-regulated in RMS3185A relative to that in RMS3185B. Besides, genes involved in ko04626, including twoCMLand one transcription factor WRKY33, were up-regulated in RMS3185A relative to that in RMS3185B. In conclusion, we hypothesized that the expression alterations of these genes were responsible for calcium signaling and sugar metabolism, thus affecting the occurrence of RGMS in cabbage.