Induction of T lymphocytes specific for bovine viral diarrhea virus in calves with maternal antibody
Induction of T lymphocytes specific for bovine viral diarrhea virus in calves with maternal antibody
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DOI：
10.1089/088282404322875421
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发表时间：
2004-01-01
期刊：
影响因子：
2.2
通讯作者：
Roth, JA
中科院分区：
文献类型：
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作者：
Endsley, JJ;Ridpath, JF;Roth, JA
通过摄入初乳获得的牛病毒性腹泻病毒（BVDV）的被动抗体可能会干扰小牛对该病毒的保护性免疫应答。本研究的目的是确定具有高水平牛病毒性腹泻病毒（BVDV）母源抗体的小牛在不存在可测量的体液免疫应答的情况下是否对BVDV产生CD 4（+）、CD 8（+）或γ δ T淋巴细胞应答。在2-5周龄时和/或母源抗体减弱后，用强毒BVDV攻毒初乳或牛奶添加剂喂养的小牛。当被动抗体水平较高时，暴露于BVDV的小牛未产生可测量的BVDV体液免疫应答。然而，与未暴露动物相比，这些动物在体外暴露于BVDV后，其CD 4（+）、CD 8（+）和γ δ T淋巴细胞被BVDV激活。与未暴露小牛相比，在存在母体抗体的情况下，暴露于BVDV的小牛的淋巴细胞在体外BVDV暴露后产生的IFN γ也更高。这些数据表明，当母源抗体水平较高时，暴露于BVDV的小牛可在无主动抗体应答的情况下产生抗原特异性CD 4（+）、CD 8（+）和γ δ T淋巴细胞。单独提供的手稿证明，本研究中具有BVDV特异性T淋巴细胞的小牛在母体抗体不可检出后，也可免受强毒BVDV基因型2攻毒。
Passive antibody to bovine viral diarrhea virus (BVDV) acquired through colostrum intake may interfere with the development of a protective immune response by calves to this virus. The objective of this study was to determine if calves, with a high level of maternal antibody to bovine viral diarrhea virus (BVDV), develop CD4(+), CD8(+), or gammadelta T lymphocyte responses to BVDV in the absence of a measurable humoral immune response. Colostrum or milk replacer fed calves were challenged with virulent BVDV at 2-5 weeks of age and/or after maternal antibody had waned. Calves exposed to BVDV while passive antibody levels were high did not mount a measurable humoral immune response to BVDV. However, compared to nonexposed animals, these animals had CD4(+), CD8(+), and gammadelta T lymphocytes that were activated by BVDV after exposure to in vitro BVDV. The production of IFNgamma by lymphocytes after in vitro BVDV exposure was also much greater in lymphocytes from calves exposed to BVDV in the presence of maternal antibody compared to the nonexposed calves. These data indicate that calves exposed to BVDV while maternal antibody levels are high can develop antigen specific CD4(+), CD8(+), and gammadelta T lymphocytes in the absence of an active antibody response. A manuscript presented separately demonstrates that the calves with T lymphocytes specific for BVDV in this study were also protected from virulent BVDV genotype 2 challenge after maternal antibody became undetectable.