Factor-independent transcription pausing caused by recognition of the RNA-DNA hybrid sequence.
Factor-independent transcription pausing caused by recognition of the RNA-DNA hybrid sequence.
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DOI：
10.1038/emboj.2011.432
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发表时间：
2012-02-01
期刊：
影响因子：
11.4
通讯作者：
Zenkin, Nikolay
中科院分区：
文献类型：
--
作者：
Bochkareva, Aleksandra;Yuzenkova, Yulia;Tadigotla, Vasisht R.;Zenkin, Nikolay
转录暂停是细菌和真核生物基因表达调控的重要步骤。在这里，我们发现了一个独立的转录暂停机制，这是由延伸RNA聚合酶识别RNA-DNA杂交序列的能力所决定的。我们表明，独立的热力学稳定性的延伸复合物，RNA聚合酶直接“感觉”的形状和/或身份的RNA-DNA杂交的碱基对。RNA-DNA杂交序列的识别延迟了RNA聚合酶的易位，从而通过“途径内”机制减慢了核苷酸添加循环。我们发现，这种现象是保守的细菌和真核生物的RNA聚合酶，并参与监管暂停，如暂停调节生产的毒力因子在某些细菌和暂停调节HIV-1的转录/复制。结果表明，多亚基RNA聚合酶对RNA-DNA杂交序列的识别参与转录调控，并可能决定转录延伸的总体速率。
Pausing of transcription is an important step of regulation of gene expression in bacteria and eukaryotes. Here we uncover a factor-independent mechanism of transcription pausing, which is determined by the ability of the elongating RNA polymerase to recognize the sequence of the RNA–DNA hybrid. We show that, independently of thermodynamic stability of the elongation complex, RNA polymerase directly ‘senses’ the shape and/or identity of base pairs of the RNA–DNA hybrid. Recognition of the RNA–DNA hybrid sequence delays translocation by RNA polymerase, and thus slows down the nucleotide addition cycle through ‘in pathway’ mechanism. We show that this phenomenon is conserved among bacterial and eukaryotic RNA polymerases, and is involved in regulatory pauses, such as a pause regulating the production of virulence factors in some bacteria and a pause regulating transcription/replication of HIV-1. The results indicate that recognition of RNA–DNA hybrid sequence by multi-subunit RNA polymerases is involved in transcription regulation and may determine the overall rate of transcription elongation.
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影响因子：
3.6
作者：
Hatoum, Asma;Roberts, Jeffrey
通讯作者：
Roberts, Jeffrey
影响因子：
3.4
作者：
Maoileidigh, Daibhid O.;Tadigotla, Vasisht R.;Ruckenstein, Andrei E.
通讯作者：
Ruckenstein, Andrei E.
影响因子：
2.9
作者：
Hsu, Lilian M.;Cobb, Ingrid M.;Ahn, Colette
通讯作者：
Ahn, Colette
影响因子：
14.9
作者：
FARNHAM, PJ;PLATT, T
通讯作者：
PLATT, T
DOI：
10.1073/pnas.0900407106
发表时间：
2009-06-02
影响因子：
11.1
作者：
Kireeva, Maria L.;Kashlev, Mikhail
通讯作者：
Kashlev, Mikhail