Fast kinetic measurements and on-line dilution by flow injection cytometry.
Fast kinetic measurements and on-line dilution by flow injection cytometry.
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通过流式注射细胞术进行快速动力学测量和在线稀释。
DOI：
10.1002/cyto.990160406
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Christian,GD
中科院分区：
文献类型：
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作者：
Lindberg,W;Scampavia,LD;Ruzicka,J;Christian,GD
描述了一种改进的流式注射细胞术（FIC）系统，适用于快速细胞动力学测量和在线稀释。该仪器允许在混合开始后 1.2 秒（± 0.05 秒）内直至此后的任何时间进行测量。评估和讨论了确定快速动力学测量的关键因素，例如样品引入的位移体积、传输到细胞计数询问点的速率以及混合速度。通过改变吸入的体积，该仪器可以促进细胞和/或试剂在一个数量级的范围内的稀释。通过使用 4', 6-二脒基-2-苯基吲哚 (DAPI) 对鳟鱼红细胞中的 DNA 进行在线染色，证明了快速动力学和在线稀释能力。使用人类淋巴细胞测量谷胱甘肽 S-转移酶 (GST) 催化的一氯代二甲烷 (MCB) 和谷胱甘肽 (GSH) 之间的反应，说明了该仪器用于测量酶动力学的实用性。 © 1994 Wiley-Liss, Inc.
An improved flow injection cytometry (FIC) system suitable for fast cellular kinetic measurements and on‐line dilution is described. The instrument allows for measurements from 1.2 s (± 0.05 s) after the initiation of mixing, up to any time thereafter. Crucial factors in determining fast kinetic measurements, such as the displacement volume for sample introduction, rate of transport to cytometric interrogation point, and the mixing speed are evaluated and discussed. By varying the volume aspirated, this instrument can facilitate the dilution of cells and/or reagent over a range of one order of magnitude. The fast kinetic and on‐line dilution capabilities were demonstrated by on‐line staining of DNA in trout erythrocytes with 4′, 6‐diamidino‐2‐phenylindole (DAPI). The utility of the instrument for measurement of enzyme kinetics was illustrated using human lymphocytes, measuring the glutathione S‐transferase (GST) catalyzed reaction between monochlorobimane (MCB) and glutathione (GSH). © 1994 Wiley‐Liss, Inc.