Neuroprotection by hyperbaric oxygenation after experimental focal cerebral ischemia monitored by MRI
Neuroprotection by hyperbaric oxygenation after experimental focal cerebral ischemia monitored by MRI
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DOI：
10.1161/01.str.0000125868.86298.8e
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发表时间：
2004-05-01
期刊：
影响因子：
8.3
通讯作者：
Schwab, S
中科院分区：
文献类型：
--
作者：
Schäbitz, WR;Schade, H;Schwab, S
背景：脑缺血后早期应用高压氧（HBO）可减少梗死面积并改善预后。在这里，我们评估了HBO对永久性局灶性脑缺血的影响，并应用磁共振成像（MRI）监测研究病变evolution. Methods大鼠进行永久性大脑中动脉闭塞（MCAO）。两小时后，动物用HBO（100%O-2/2atm; n=17）处理1小时或用室内空气处理（n=17）。动物在MCAO后90分钟开始接受系列MRI研究（DWI、PI、T2）。评估神经评分（5分评定量表）。在MCAO后120小时，对动物实施安乐死，并对脑进行2，3，5-氯化三苯基四唑（TTC）染色以计算梗死体积。用抗c-FOS和4-羟基-2-壬烯醛修饰蛋白（HNE）的抗体进行免疫组织化学，以检查HBO治疗引起的氧化应激的影响。
Background-Hyperbaric oxygenation (HBO) after focal cerebral ischemia reduces infarct size and improves outcome when applied early after stroke. Here, we evaluated effects of HBO on permanent focal cerebral ischemia and applied magnetic resonance imaging (MRI) monitoring to study lesion evolution.Methods-Rats underwent permanent middle cerebral artery occlusion (MCAO). Two hours later, animals were treated with HBO (100% O-2/2 atm; n=17) for 1 hour or treated with room air (n=17). Animals underwent serial MRI studies (DWI, PI, T2) beginning 90 minutes after MCAO. Neuroscore was assessed (5-point rating scale). Animals were euthanized and brains were 2,3,5-triphenyltetrazolium chloride (TTC)-stained for infarct volume calculation 120 hours after MCAO. Immunohistochemistry was performed with antibodies against c-FOS and 4-hydroxy-2-nonenal-modified proteins (HNE) to check for effects of oxidative stress caused by HBO treatment.Results-HBO reduced infarct volume by 38% (P